Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 770 68.5 3470224 91.7% 3182195 103.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,624,357 (T)8→7 coding (141/393 nt) ydeI ← hydrogen peroxide resistance OB fold protein, putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,624,3500T.100.0% 257.2 / NA 63coding (148/393 nt)ydeIhydrogen peroxide resistance OB fold protein, putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (33/30);  total (33/30)

CCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATA  >  NC_000913/1624215‑1624478
                                                                                                                                       |                                                                                                                                
ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAtttt                                                                                                                               >  1:1424191/1‑139 (MQ=255)
          aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    <  2:1463544/139‑1 (MQ=255)
               cATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGtt                                                                                                               <  2:633817/139‑1 (MQ=255)
                aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc                                                                                                              >  1:665201/1‑139 (MQ=255)
                aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGAGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc                                                                                                              >  1:1198378/1‑139 (MQ=255)
                  tAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCcg                                                                                                            >  1:652079/1‑139 (MQ=255)
                          ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    <  2:461807/123‑1 (MQ=255)
                          ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    >  1:461807/1‑123 (MQ=255)
                              tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    <  2:1603406/119‑1 (MQ=255)
                              tctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    >  1:1603406/1‑119 (MQ=255)
                                  aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                            >  1:1557638/1‑139 (MQ=255)
                                     gcgAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGATTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTg                                                                                         >  2:1469750/1‑139 (MQ=255)
                                                      ccTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgc                                                                        <  2:665201/139‑1 (MQ=255)
                                                       ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                       <  2:4934/139‑1 (MQ=255)
                                                       ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                       >  2:970182/1‑139 (MQ=255)
                                                        tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc                                                                                                              <  2:1540109/99‑1 (MQ=255)
                                                        tctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTc                                                                                                              >  1:1540109/1‑99 (MQ=255)
                                                         ctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                     <  2:1557638/139‑1 (MQ=255)
                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                    <  2:264667/102‑1 (MQ=255)
                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGcc                                                                                                    >  1:264667/1‑102 (MQ=255)
                                                               tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                               >  1:240804/1‑139 (MQ=255)
                                                                   agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGtt                                                                                                               >  2:1634636/1‑87 (MQ=255)
                                                                   agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGtt                                                                                                               <  1:1634636/87‑1 (MQ=255)
                                                                   agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGa                                                                                                         >  1:401319/1‑93 (MQ=255)
                                                                   agaAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGa                                                                                                         <  2:401319/93‑1 (MQ=255)
                                                                      aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    <  1:20148/79‑1 (MQ=255)
                                                                      aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    >  2:20148/1‑79 (MQ=255)
                                                                      aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGtt                                                                                                               >  1:1251095/1‑84 (MQ=255)
                                                                      aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGtt                                                                                                               <  2:1251095/84‑1 (MQ=255)
                                                                       aTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaa                                                       >  2:576190/1‑139 (MQ=255)
                                                                        tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                      >  2:120432/1‑139 (MQ=255)
                                                                         cAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                     <  1:970182/139‑1 (MQ=255)
                                                                              tACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGccc                                                <  2:528314/139‑1 (MQ=255)
                                                                                  gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                            >  2:1077254/1‑139 (MQ=255)
                                                                                   cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    <  1:986849/66‑1 (MQ=255)
                                                                                   cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGcatcat                                                                                                                    >  2:986849/1‑66 (MQ=255)
                                                                                   cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                           <  2:1424191/139‑1 (MQ=255)
                                                                                   cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                           <  1:576190/139‑1 (MQ=255)
                                                                                      aGTGAAACCGAAGCGCCATCGTGCATGGATTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                        <  1:1469750/139‑1 (MQ=255)
                                                                                          aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                    <  2:652079/139‑1 (MQ=255)
                                                                                           aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                   <  2:272700/139‑1 (MQ=255)
                                                                                           aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                   <  1:120432/139‑1 (MQ=255)
                                                                                           aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                   <  2:1198378/139‑1 (MQ=255)
                                                                                                aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                          >  2:801118/1‑46 (MQ=255)
                                                                                                aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAtttttttgc                                                                                                                          <  1:801118/48‑3 (MQ=255)
                                                                                                aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                              >  1:134136/1‑139 (MQ=255)
                                                                                                aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                              >  2:833738/1‑139 (MQ=255)
                                                                                                aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                              >  2:980510/1‑139 (MQ=255)
                                                                                                 aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                            >  1:841555/1‑124 (MQ=255)
                                                                                                 aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                            <  2:841555/124‑1 (MQ=255)
                                                                                                  gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                >  2:500283/1‑103 (MQ=255)
                                                                                                  gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                <  1:500283/103‑1 (MQ=255)
                                                                                                  gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                            >  1:1254308/1‑139 (MQ=255)
                                                                                                  gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                            >  1:231922/1‑139 (MQ=255)
                                                                                                  gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTACATc                                                                >  1:1427167/1‑103 (MQ=255)
                                                                                                  gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTACATc                                                                <  2:1427167/103‑1 (MQ=255)
                                                                                                                ggTTTTTGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa              >  1:472351/1‑139 (MQ=255)
                                                                                                                  tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa            >  2:525008/1‑139 (MQ=255)
                                                                                                                  tttttGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa            <  2:240804/139‑1 (MQ=255)
                                                                                                                      tGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                <  2:546931/83‑1 (MQ=255)
                                                                                                                      tGCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                >  1:546931/1‑83 (MQ=255)
                                                                                                                       gCGAAATCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGc       <  1:980510/139‑1 (MQ=255)
                                                                                                                          aaaTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGa                                                                                                         >  1:993585/1‑38 (MQ=37)
                                                                                                                          aaaTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGa                                                                                                         <  2:993585/38‑1 (MQ=37)
                                                                                                                           aaTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAAt   >  2:513492/1‑139 (MQ=255)
                                                                                                                            aTCAACGGTCA‑TTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATa  >  2:1586554/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                
CCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTTTTTTTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATA  >  NC_000913/1624215‑1624478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A