Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,346,086 |
A→G |
I414T (ATC→ACC) |
fumB ← |
anaerobic class I fumarate hydratase (fumarase B) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,346,086 | 0 | A | G | 98.0%
| 178.0
/ ‑6.7
| 51 | I414T (ATC→ACC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/25); minor base C (0/1); total (25/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGA > NC_000913/4345950‑4346184
|
gATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTg > 1:873510/1‑139 (MQ=255)
gATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTg < 2:873510/139‑1 (MQ=255)
tCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTa < 2:682014/139‑1 (MQ=255)
gccgccGTGGGATTGAAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGTGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGa > 2:620125/1‑139 (MQ=255)
aTCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATc > 2:641940/1‑139 (MQ=255)
ccACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg > 1:738272/1‑115 (MQ=255)
ccACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg < 2:738272/115‑1 (MQ=255)
gTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGc > 1:48137/1‑139 (MQ=255)
cATACGGCCTGCGGTGGTTGGGCCGAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATGGGGTGATCTTTGGTGTa < 2:533336/96‑1 (MQ=38)
cATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTa > 1:533336/1‑96 (MQ=255)
aTACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTgg > 1:882775/1‑139 (MQ=255)
tGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCa < 1:175992/139‑1 (MQ=255)
ggtggtTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAata > 2:747583/1‑139 (MQ=255)
ggtTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCATCTTGGCGTGTGCAATAtct < 1:620125/139‑1 (MQ=255)
ggtTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATAtct < 1:641940/139‑1 (MQ=255)
ttGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAg > 2:723090/1‑88 (MQ=255)
ttGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAg < 1:723090/88‑1 (MQ=255)
ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc < 2:126648/139‑1 (MQ=255)
gCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTg > 1:403609/1‑73 (MQ=255)
gCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTg < 2:403609/73‑1 (MQ=255)
gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGc < 1:647632/110‑1 (MQ=255)
gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGc > 2:647632/1‑110 (MQ=255)
gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt > 1:765325/1‑139 (MQ=255)
tAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTAc > 2:606433/1‑57 (MQ=255)
tAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTAc < 1:606433/57‑1 (MQ=255)
tAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGa > 1:143134/1‑139 (MQ=255)
aaCCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAg > 2:172481/1‑139 (MQ=255)
cGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCtt > 1:291786/1‑94 (MQ=255)
cGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCtt < 2:291786/94‑1 (MQ=255)
ggCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATaa > 2:90697/1‑122 (MQ=255)
ggCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATaa < 1:90697/122‑1 (MQ=255)
gCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACa < 2:48137/139‑1 (MQ=255)
gCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACa < 1:747583/139‑1 (MQ=255)
gCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACa < 1:172481/139‑1 (MQ=255)
ggggTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAAc < 1:647525/139‑1 (MQ=255)
tagATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACaaa > 2:543477/1‑114 (MQ=255)
tagATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACaaa < 1:543477/114‑1 (MQ=255)
cGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt > 2:345501/1‑53 (MQ=255)
cGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt < 1:345501/53‑1 (MQ=255)
cGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTg > 1:764934/1‑100 (MQ=255)
cGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTg < 2:764934/100‑1 (MQ=255)
ggTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc > 1:543287/1‑79 (MQ=255)
ggTGATCTTTGCTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc < 2:543287/79‑1 (MQ=255)
gTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc < 1:882835/78‑1 (MQ=255)
gTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc > 2:882835/1‑78 (MQ=255)
gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc > 2:629094/1‑58 (MQ=255)
gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc < 1:629094/58‑1 (MQ=255)
gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAg < 2:125453/97‑1 (MQ=255)
gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAg > 1:125453/1‑97 (MQ=255)
gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGa < 1:62114/107‑1 (MQ=255)
gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGa > 2:62114/1‑107 (MQ=255)
|
GATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGA > NC_000913/4345950‑4346184
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
