Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I1 R1 759 65.2 3386260 90.7% 3071337 101.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,346,086 A→G I414T (ATC→ACC)  fumB ← anaerobic class I fumarate hydratase (fumarase B)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,346,0860AG100.0% 191.1 / NA 54I414T (ATC→ACC) fumBanaerobic class I fumarate hydratase (fumarase B)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

TGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGAT  >  NC_000913/4345958‑4346219
                                                                                                                                |                                                                                                                                     
tgcCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg                                                                                                                             <  1:203184/139‑1 (MQ=255)
  ccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGaa                                                                                                                           >  2:319888/1‑139 (MQ=255)
                       tCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCa                                                                                                      <  2:818795/139‑1 (MQ=255)
                                gAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCa                                                                                             >  2:707797/1‑139 (MQ=255)
                                  gTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGc                                                                                           >  2:1179173/1‑139 (MQ=255)
                                     cATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTAc                                                                                                                  >  2:303599/1‑113 (MQ=255)
                                     cATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTAc                                                                                                                  <  1:303599/113‑1 (MQ=255)
                                     cATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg                                                                                        <  1:1179173/139‑1 (MQ=255)
                                     cATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg                                                                                        >  2:468394/1‑139 (MQ=255)
                                       tACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg                                                                                                                             >  1:345094/1‑100 (MQ=255)
                                       tACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg                                                                                                                             <  2:345094/100‑1 (MQ=255)
                                       tACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc                                                                                      <  1:833921/139‑1 (MQ=255)
                                         cGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCgt                                                                                    <  2:537353/139‑1 (MQ=255)
                                          ggCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCgtg                                                                                   <  1:1176395/139‑1 (MQ=255)
                                            ccTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGtgtg                                                                                 >  2:32665/1‑139 (MQ=255)
                                             cTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAg                                                                                                     <  2:980100/118‑1 (MQ=255)
                                             cTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAg                                                                                                     >  1:980100/1‑118 (MQ=255)
                                             cTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGc                                                                                >  1:1482636/1‑139 (MQ=255)
                                                      ttGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCg                                                                       >  2:1312373/1‑139 (MQ=255)
                                                         ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                    <  1:319888/139‑1 (MQ=255)
                                                               gTGAACCTGCTGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTACGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg                                                                                        <  1:1019581/113‑1 (MQ=255)
                                                               gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt                                                              >  2:274044/1‑139 (MQ=255)
                                                               gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTACGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg                                                                                        >  2:1019581/1‑113 (MQ=255)
                                                                 gAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATaa                                                            <  2:1482636/139‑1 (MQ=255)
                                                                   aCCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATg                                                          <  1:32665/139‑1 (MQ=255)
                                                                    ccTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                    >  2:334872/1‑128 (MQ=255)
                                                                    ccTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                    <  1:334872/128‑1 (MQ=255)
                                                                    ccTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                    >  2:901705/1‑128 (MQ=255)
                                                                    ccTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAAATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                    <  1:901705/128‑1 (MQ=255)
                                                                         tGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCg                                                    >  1:990701/1‑139 (MQ=255)
                                                                         tGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAG‑TCGGGTGATCTTCGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCgg                                                   <  1:1118539/139‑1 (MQ=255)
                                                                             tAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                    <  1:467646/119‑1 (MQ=255)
                                                                             tAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                    >  2:467646/1‑119 (MQ=255)
                                                                                  ggCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTAc                                                                                                                  <  2:308184/68‑1 (MQ=255)
                                                                                  ggCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTAc                                                                                                                  >  1:308184/1‑68 (MQ=255)
                                                                                  ggCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGAc                                           >  1:1139696/1‑139 (MQ=255)
                                                                                   gCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCg                                                                                                             <  2:279759/72‑1 (MQ=255)
                                                                                   gCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCg                                                                                                             >  1:279759/1‑72 (MQ=255)
                                                                                     aGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGATGTTCTTTACCGGCGTCAATCAGCTCTTTCAGc                                                                                           <  2:1004493/88‑1 (MQ=255)
                                                                                     aGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTGACCGGCGTCAATCAGCTCTTTCAGc                                                                                           >  1:1004493/1‑88 (MQ=255)
                                                                                          ttttCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGt                                   <  1:597040/139‑1 (MQ=255)
                                                                                                 cGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt                                                                                                <  2:207450/71‑1 (MQ=255)
                                                                                                 cGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt                                                                                                >  1:207450/1‑71 (MQ=255)
                                                                                                 cGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATAcc                            <  2:990701/139‑1 (MQ=255)
                                                                                                   gACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCa                                                                                                      <  2:385808/63‑1 (MQ=255)
                                                                                                   gACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCa                                                                                                      >  1:385808/1‑63 (MQ=255)
                                                                                                                    gggTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCaa                                                                              <  1:1467854/70‑1 (MQ=255)
                                                                                                                    gggTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCaa                                                                              >  2:1467854/1‑70 (MQ=255)
                                                                                                                     ggTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGc        <  2:1139696/139‑1 (MQ=255)
                                                                                                                      gTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGc                                                                     <  2:413455/77‑1 (MQ=255)
                                                                                                                      gTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGc                                                                     >  1:413455/1‑77 (MQ=255)
                                                                                                                         aTCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAgg    <  1:707797/139‑1 (MQ=255)
                                                                                                                          tCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGa   >  2:31171/1‑139 (MQ=255)
                                                                                                                           cTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGAt  >  2:1394650/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                     
TGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGAT  >  NC_000913/4345958‑4346219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A