Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,346,086 |
A→G |
I414T (ATC→ACC) |
fumB ← |
anaerobic class I fumarate hydratase (fumarase B) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,346,086 | 0 | A | G | 100.0%
| 94.0
/ NA
| 28 | I414T (ATC→ACC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/15); total (13/15) |
CATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGA > NC_000913/4345953‑4346215
|
cATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGTGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTAc < 1:61893/139‑1 (MQ=255)
tgcCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg < 2:33310/139‑1 (MQ=255)
tAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt > 1:554693/1‑139 (MQ=255)
tCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCt > 1:934872/1‑139 (MQ=255)
tCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCt > 1:845992/1‑139 (MQ=255)
aCGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCg < 1:898456/139‑1 (MQ=255)
ccTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGtgtg < 2:275574/139‑1 (MQ=255)
gggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGc < 2:845992/139‑1 (MQ=255)
gCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTAGCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATAtct > 1:664040/1‑133 (MQ=255)
gCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATAtct < 2:664040/133‑1 (MQ=255)
gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGgtgt < 1:1039807/69‑1 (MQ=255)
gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGgtgt > 2:1039807/1‑69 (MQ=255)
gAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATaa < 1:287603/139‑1 (MQ=255)
aaCCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAg < 1:546577/139‑1 (MQ=255)
aCCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCg > 1:253355/1‑100 (MQ=255)
aCCGGCAGGGGTTTTCGCCGAACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCg < 2:253355/100‑1 (MQ=255)
ttttCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCaa < 1:285001/96‑1 (MQ=255)
ttttCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCaa > 2:285001/1‑96 (MQ=255)
ttCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCttt > 1:608749/1‑77 (MQ=255)
ttCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCttt < 2:608749/77‑1 (MQ=255)
ttCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCg < 1:387486/87‑1 (MQ=255)
ttCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCg > 2:387486/1‑87 (MQ=255)
cGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg < 2:23041/79‑1 (MQ=255)
cGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg > 1:23041/1‑79 (MQ=255)
cgTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTg > 1:355776/1‑139 (MQ=255)
gTGATCTTTGGTGTATTGCGGAAGTTCTTTACCGGCGTCACTCAGCTCTTCCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCg < 2:316488/139‑1 (MQ=255)
gTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATg > 2:581622/1‑88 (MQ=255)
gTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATg < 1:581622/88‑1 (MQ=255)
gTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCg > 1:483427/1‑139 (MQ=255)
tGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGa > 1:936814/1‑139 (MQ=255)
|
CATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGA > NC_000913/4345953‑4346215
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
