Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,346,086	A→G	I414T (ATC→ACC)	fumB ←	anaerobic class I fumarate hydratase (fumarase B)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,346,086	0	A	G	100.0%	164.7 / NA	47	I414T (ATC→ACC)	fumB	anaerobic class I fumarate hydratase (fumarase B)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/25); total (22/25)

GCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATC  >  NC_000913/4345959‑4346220

gccgccGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGa                                                                                                                             <  1:85993/139‑1 (MQ=255)

 ccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGaa                                                                                                                            >  2:521330/1‑139 (MQ=255)

                     aTCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATc                                                                                                        >  1:334212/1‑139 (MQ=255)

                       ccACGTAGGAGTCCATACGGCCTGCGGTGCTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGGAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAg                                                                                                      <  1:521330/139‑1 (MQ=255)

                                  tCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCt                                                                                           >  2:75261/1‑139 (MQ=255)

                                  tCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCt                                                                                           >  2:163010/1‑139 (MQ=255)

                                   ccATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg                                                                                                                              >  2:580723/1‑103 (MQ=255)

                                   ccATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCgg                                                                                                                              <  1:580723/103‑1 (MQ=255)

                                    cATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg                                                                                         >  1:95372/1‑139 (MQ=255)

                                    cATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTg                                                                                         <  1:570358/139‑1 (MQ=255)

                                      tACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc                                                                                       <  2:95372/139‑1 (MQ=255)

                                      tACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc                                                                                       <  2:668286/139‑1 (MQ=255)

                                       aCGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCg                                                                                      >  1:114120/1‑139 (MQ=255)

                                        cGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCgt                                                                                     <  1:172412/139‑1 (MQ=255)

                                           ccTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGtgtg                                                                                  >  1:13134/1‑139 (MQ=255)

                                            cTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGc                                                                                 >  1:122588/1‑139 (MQ=255)

                                                   ggtTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATAtct                                                                          <  1:616091/139‑1 (MQ=255)

                                                     ttGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCg                                                                        <  2:334212/139‑1 (MQ=255)

                                                       gggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt                                                                                                 >  2:557531/1‑112 (MQ=255)

                                                       gggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt                                                                                                 <  1:557531/112‑1 (MQ=255)

                                                        ggCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGcc                                                                     <  1:690612/139‑1 (MQ=255)

                                                         gCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGccc                                                                    >  2:436268/1‑139 (MQ=255)

                                                              gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt                                                               >  1:675174/1‑139 (MQ=255)

                                                              gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt                                                               >  2:6979/1‑139 (MQ=255)

                                                              gTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt                                                               >  2:355691/1‑139 (MQ=255)

                                                                        tGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc                                                                                       >  2:767947/1‑105 (MQ=255)

                                                                        tGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGc                                                                                       <  1:767947/105‑1 (MQ=255)

                                                                           aTAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTg                                                  <  1:75261/139‑1 (MQ=255)

                                                                           aTAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTg                                                  <  1:418439/139‑1 (MQ=255)

                                                                                  gCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACa                                           <  1:436268/139‑1 (MQ=255)

                                                                                    aGGGGCTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACaaa                                         <  1:479166/139‑1 (MQ=255)

                                                                                      gggTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg                                       <  2:106750/139‑1 (MQ=255)

                                                                                             cGCCGGACCCACGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGAt                                <  2:500118/139‑1 (MQ=255)

                                                                                                   aCCCGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCggg                          >  2:302708/1‑139 (MQ=255)

                                                                                                    cccGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGt                         <  2:536878/139‑1 (MQ=255)

                                                                                                    cccGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGt                         <  1:622174/139‑1 (MQ=255)

                                                                                                    cccGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGt                         <  1:355691/139‑1 (MQ=255)

                                                                                                     ccGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAACTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTg                                                        >  2:713227/1‑107 (MQ=255)

                                                                                                     ccGCGTAGTAGATCGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAACTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTg                                                        <  1:713227/107‑1 (MQ=255)

                                                                                                                  cGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTgg           >  1:635625/1‑139 (MQ=255)

                                                                                                                  cGGGTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTgg           >  2:400935/1‑139 (MQ=255)

                                                                                                                    ggTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGt                                    <  2:182389/112‑1 (MQ=255)

                                                                                                                    ggTGATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGt                                    >  1:182389/1‑112 (MQ=255)

                                                                                                                       gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGt                         <  2:755677/120‑1 (MQ=255)

                                                                                                                       gATCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGt                         >  1:755677/1‑120 (MQ=255)

                                                                                                                        aTCTTTGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAgg     <  2:122588/139‑1 (MQ=255)

                                                                                                                           tttGGTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGAtc  >  1:512704/1‑139 (MQ=255)

GCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAACCGGCAGGGGTTTTCGCCGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACGAGTGGATACCGGGTATTGCGAAAGCTGGGCGAGGATC  >  NC_000913/4345959‑4346220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A