Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I1 R1 759 65.2 3386260 90.7% 3071337 101.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 812,947 G→A *226* (TAG→TAA)  bioD → dethiobiotin synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913812,9470GA98.4% 196.9 / NA 62*226* (TAG→TAA) bioDdethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/30);  minor base T (0/1);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAG  >  NC_000913/812841‑813079
                                                                                                          |                                                                                                                                    
cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCgaga                                                                                                      >  1:463257/1‑139 (MQ=255)
    cACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATcgc                                                                                                  <  2:909850/139‑1 (MQ=255)
    cACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATcgc                                                                                                  <  1:1227436/139‑1 (MQ=255)
    cACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATcgc                                                                                                  <  2:463257/139‑1 (MQ=255)
        cGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCt                                                                                              <  2:929354/139‑1 (MQ=255)
          cATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTc                                                                                            >  2:1477518/1‑139 (MQ=255)
                 cccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                                     <  1:1581164/139‑1 (MQ=255)
                          ctgctgGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTa                                                                            >  1:846094/1‑139 (MQ=255)
                          ctgctgGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTa                                                                            >  2:20391/1‑139 (MQ=255)
                                  agagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                                    <  1:20391/139‑1 (MQ=255)
                                      aTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                        <  2:979383/115‑1 (MQ=255)
                                      aTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                        >  1:979383/1‑115 (MQ=255)
                                       tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAAt                                                                               <  2:608932/123‑1 (MQ=255)
                                       tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAAt                                                                               >  1:608932/1‑123 (MQ=255)
                                       tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTc                                                               >  2:1654259/1‑139 (MQ=255)
                                          ccTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTGACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                                >  1:1531584/1‑103 (MQ=255)
                                          ccTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTGACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                                <  2:1531584/103‑1 (MQ=255)
                                             ggCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAaat                                                         >  1:76058/1‑139 (MQ=255)
                                              gCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATTGCGTCTTCGATTGAc                                                                                     <  2:705494/110‑1 (MQ=255)
                                              gCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATTGCGTCTTCGATTGAc                                                                                     >  1:705494/1‑110 (MQ=255)
                                                ttGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAaataat                                                      <  2:846094/139‑1 (MQ=255)
                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                    <  1:1014196/92‑1 (MQ=255)
                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                    >  2:1014196/1‑92 (MQ=255)
                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                                    <  1:80534/124‑1 (MQ=255)
                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                                    >  2:80534/1‑124 (MQ=255)
                                                  gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCa                                                    >  1:383957/1‑139 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTAtt                                                                                                                          >  1:1251615/1‑68 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTAtt                                                                                                                          <  2:1251615/68‑1 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTg                                                                                                               >  1:748880/1‑79 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTg                                                                                                               <  2:748880/79‑1 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGTGAGCGAGATCGCGTCTTCGAtt                                                                                        >  1:641164/1‑102 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGTGAGCGAGATCGCGTCTTCGAtt                                                                                        <  2:641164/102‑1 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                        <  1:1210462/102‑1 (MQ=255)
                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAACTGCGAGCGAGATCGCGTCTTCGAtt                                                                                        >  2:1210462/1‑102 (MQ=255)
                                                        aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTa                                                                                                                    <  1:1608776/69‑1 (MQ=255)
                                                        aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTa                                                                                                                    >  2:1608776/1‑69 (MQ=255)
                                                           ccAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgagcga                                                                                                        <  1:1155284/78‑1 (MQ=255)
                                                           ccAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgagcga                                                                                                        >  2:1155284/1‑78 (MQ=255)
                                                                   tGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTATCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACtt                                   <  1:1312196/139‑1 (MQ=255)
                                                                   tGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgag                                                                                                           <  1:477287/67‑1 (MQ=255)
                                                                   tGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgag                                                                                                           >  2:477287/1‑67 (MQ=255)
                                                                   tGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                                    >  1:884004/1‑90 (MQ=255)
                                                                   tGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                                    <  2:884004/90‑1 (MQ=255)
                                                                    gCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAt                                                                                         <  2:635026/84‑1 (MQ=255)
                                                                    gCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAt                                                                                         >  1:635026/1‑84 (MQ=255)
                                                                         aaCCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcga                                                                                                            <  2:1162627/60‑1 (MQ=255)
                                                                         aaCCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcga                                                                                                            >  1:1162627/1‑60 (MQ=255)
                                                                           ccGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCt                                                                                                                               <  1:48852/39‑1 (MQ=255)
                                                                           ccGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCt                                                                                                                               >  2:48852/1‑39 (MQ=255)
                                                                                aaGTACATAAACCTTGCCTTGTTGTAACCATGCTGTATTTGGTTAAATTGGGAGCGAGATCGCGTCTTCGATTGACTGCGATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcgg                      <  2:815107/139‑1 (MQ=255)
                                                                                 aGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                     <  2:76058/139‑1 (MQ=255)
                                                                                  gTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggcg                    <  1:1654259/139‑1 (MQ=255)
                                                                                        aaaCCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTTCGCCTCATGCGGCGAAtgtg              >  1:423794/1‑139 (MQ=255)
                                                                                         aaCCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                                     >  1:1496131/1‑67 (MQ=255)
                                                                                         aaCCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                                     <  2:1496131/67‑1 (MQ=255)
                                                                                             ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAAtt                                                                     <  1:1610559/79‑1 (MQ=255)
                                                                                             ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAAtt                                                                     >  2:1610559/1‑79 (MQ=255)
                                                                                             ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAt                                                                 >  2:287348/1‑83 (MQ=255)
                                                                                             ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAt                                                                 <  1:287348/83‑1 (MQ=255)
                                                                                             ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt         >  2:1189306/1‑139 (MQ=255)
                                                                                                    gttgtAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAg  >  2:232038/1‑139 (MQ=255)
                                                                                                    gttgtAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAg  >  1:883218/1‑139 (MQ=255)
                                                                                                          |                                                                                                                                    
CGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAG  >  NC_000913/812841‑813079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A