Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,947	G→A	226 (TAG→TAA)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,947	0	G	A	100.0%	117.3 / NA	37	226 (TAG→TAA)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/17); total (20/17)

GAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGG  >  NC_000913/812814‑813080

gAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCAtt                                                                                                                                    <  2:776038/139‑1 (MQ=255)

       tCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTAtt                                                                                                                             <  1:868272/139‑1 (MQ=255)

                    atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcg                                                                                                                >  2:275988/1‑139 (MQ=255)

                         cACGCTCACCCGCATGATTGCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgagc‑‑ga                                                                                                         >  1:702609/1‑139 (MQ=255)

                         cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTgcgagc‑‑ga                                                                                                         >  1:802719/1‑139 (MQ=255)

                           cGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCAtt                                                                                                                                    <  2:624106/112‑1 (MQ=255)

                           cGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCAtt                                                                                                                                    >  1:624106/1‑112 (MQ=255)

                             cTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATc                                                                                                     <  2:186783/139‑1 (MQ=255)

                             cTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATc                                                                                                     >  2:535659/1‑139 (MQ=255)

                               ctcccGCATGCTTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTGTCAGAAAATCCAGAAAATGCGTCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATcgc                                                                                                   <  2:979249/137‑1 (MQ=255)

                                aCCCGCATGATTCACGCACCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATcgcg                                                                                                  >  1:660075/1‑139 (MQ=255)

                                 cccGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGt                                                                                                 <  1:597539/139‑1 (MQ=255)

                                 cccGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGt                                                                                                 >  1:483719/1‑139 (MQ=255)

                                      aTGATTCCCGCGCCGCTGCTGGGAGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCtt                                                                                              >  2:960867/1‑139 (MQ=255)

                                            cccGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGAc                                                                                      <  1:435263/139‑1 (MQ=255)

                                             ccGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACt                                                                                     <  2:483719/139‑1 (MQ=255)

                                                cgccgcTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCa                                                                                  >  2:699185/1‑139 (MQ=255)

                                                              gagagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAAtt                                                                      <  1:960867/139‑1 (MQ=255)

                                                                    tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTc                                                                >  2:167502/1‑139 (MQ=255)

                                                                      cccTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTa                                                              <  1:422781/139‑1 (MQ=255)

                                                                         tGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTaaaa                                                           >  1:950140/1‑139 (MQ=255)

                                                                               gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCa                                                     >  1:284312/1‑139 (MQ=255)

                                                                                cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGAc                                                                                      <  1:756230/105‑1 (MQ=255)

                                                                                cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAAGTGCGAGC‑‑GAGATCGCGTCTTCGATTGAc                                                                                      >  2:756230/1‑105 (MQ=255)

                                                                                     aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGaaaa                                               >  1:46793/1‑139 (MQ=255)

                                                                                                              agaaCATAAACCTTGCCTTGTTGTAACCTTTCTGTATTTGTTTAAATTGCGACC‑‑GAGTTCGCGTCTTCGATTGACTGCAATTAAACAAATAAAATTCTAAAATAATCCGGAAAAAAATTTTACTTCCGCCTCATgcggc                      <  1:699185/136‑1 (MQ=255)

                                                                                                              aGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                      <  2:284312/139‑1 (MQ=255)

                                                                                                               gTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggct                     <  2:660075/139‑2 (MQ=255)

                                                                                                                          ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt          >  1:283412/1‑139 (MQ=255)

                                                                                                                          ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt          >  2:855064/1‑139 (MQ=255)

                                                                                                                          ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt          >  1:52555/1‑139 (MQ=255)

                                                                                                                          ttgccttgTTGTAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt          >  2:355981/1‑139 (MQ=255)

                                                                                                                                 gttgtAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCt                                                                                               <  1:495718/47‑1 (MQ=255)

                                                                                                                                 gttgtAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCt                                                                                               >  2:495718/1‑47 (MQ=255)

                                                                                                                                  ttgtAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAgg  <  2:912174/139‑1 (MQ=255)

                                                                                                                                  ttgtAACCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAgg  <  1:1040446/139‑1 (MQ=255)

                                                                                                                                   tgtAACCATTCTGTATTTGGTTAAATTGCGAGCGAGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAg   >  2:379609/1‑139 (MQ=255)

GAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGG‑‑GAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGC‑‑GAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGG  >  NC_000913/812814‑813080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A