Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,947	G→A	226 (TAG→TAA)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,947	0	G	A	100.0%	139.4 / NA	44	226 (TAG→TAA)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/23); total (21/23)

ATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAGCCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAT  >  NC_000913/812834‑813072

atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcg                                                                                                      >  1:1245786/1‑139 (MQ=255)

  gACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcgag                                                                                                    <  2:477364/139‑1 (MQ=255)

     cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcgagcga                                                                                                 >  2:872874/1‑139 (MQ=255)

         cTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATc                                                                                             <  2:1245786/139‑1 (MQ=255)

            aCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATcgcg                                                                                          >  1:1006341/1‑139 (MQ=255)

            aCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATcgcg                                                                                          >  2:811830/1‑139 (MQ=255)

                  aTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCg                                                                                    >  2:1374658/1‑139 (MQ=255)

                     aTTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                 <  1:1374658/139‑1 (MQ=255)

                         ccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                             <  1:886029/139‑1 (MQ=255)

                          cgcgCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTg                                                                            <  1:1169747/139‑1 (MQ=255)

                              ccgcTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGGTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              <  2:482627/134‑1 (MQ=255)

                              ccgcTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGGTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              >  1:482627/1‑134 (MQ=255)

                                        gagagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAAtt                                                              <  1:890891/139‑1 (MQ=255)

                                         agagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                             <  1:714254/139‑1 (MQ=255)

                                         agagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                             <  1:811830/139‑1 (MQ=255)

                                            gaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                             <  2:339782/136‑1 (MQ=255)

                                            gaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                             >  1:339782/1‑136 (MQ=255)

                                             aTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcgagc                                                                                                   <  2:1652729/97‑1 (MQ=255)

                                             aTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcgagc                                                                                                   >  1:1652729/1‑97 (MQ=255)

                                                 ccTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCATTTAAATCCTaa                                                     <  2:1006341/139‑1 (MQ=255)

                                                      cTTGCAGAAAATCCAGGAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAaataa                                                >  2:356206/1‑139 (MQ=255)

                                                         gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcga                                                                                                     >  1:1732812/1‑83 (MQ=255)

                                                         gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcga                                                                                                     <  2:1732812/83‑1 (MQ=255)

                                                         gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTaaa                                                           >  1:1290069/1‑125 (MQ=255)

                                                         gCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTaaa                                                           <  2:1290069/125‑1 (MQ=255)

                                                          cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCAc                                            >  2:374710/1‑139 (MQ=255)

                                                               aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACC‑TTCTGTTATTTGGTTAATTTGCGAGCAAGAT‑GCGGTTTTGAATTACTTCCATTTTAACAATTAAATTTTTAAAATATTACGaaaaa                                      >  2:622102/1‑139 (MQ=255)

                                                                       aaaTGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTa                               >  2:11772/1‑139 (MQ=255)

                                                                           gCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcgag                                                                                                    >  2:1516044/1‑66 (MQ=255)

                                                                           gCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTgcgag                                                                                                    <  1:1516044/66‑1 (MQ=255)

                                                                             ggCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              >  1:379030/1‑86 (MQ=255)

                                                                             ggCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              <  2:379030/86‑1 (MQ=255)

                                                                                aaCCGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCt                      <  1:11772/139‑1 (MQ=255)

                                                                                  ccGGAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCa                    >  1:900017/1‑139 (MQ=255)

                                                                                     gAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCa                                             <  2:1381520/111‑1 (MQ=255)

                                                                                     gAAAGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCa                                             >  1:1381520/1‑111 (MQ=255)

                                                                                      aaaGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcg                <  2:877470/139‑1 (MQ=255)

                                                                                       aaGTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcgg               <  1:872874/139‑1 (MQ=255)

                                                                                         gTACATAAACCTTGCCTT‑GTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggcg             <  1:1005146/139‑1 (MQ=255)

                                                                                                    ttgcctt‑gTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              >  1:1299360/1‑63 (MQ=255)

                                                                                                    ttgcctt‑gTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                              <  2:1299360/63‑1 (MQ=255)

                                                                                                    ttgcctt‑gTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt  >  2:2312/1‑139 (MQ=255)

                                                                                                      gcctt‑gTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                 <  1:1523642/58‑1 (MQ=255)

                                                                                                      gcctt‑gTTGTAACCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                 >  2:1523642/1‑58 (MQ=255)

ATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTT‑GTTGTAGCCATTCTG‑TATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAT  >  NC_000913/812834‑813072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A