Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,138	(A)_6→7	coding (15/594 nt)	bdcR →	transcriptional repressor for divergent bdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,132	1	.	A	98.8%	254.4 / ‑3.8	80	coding (9/594 nt)	bdcR	transcriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (41/38); total (42/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTT  >  NC_000913/4473998‑4474261

ccGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aaaa                                                                                                                                >  1:1262930/1‑139 (MQ=255)

          gACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAAtctc                                                                                                                      <  1:396369/139‑1 (MQ=255)

           aCTGCCACCGAGGATGTGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATGTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     >  2:301010/1‑139 (MQ=255)

           aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     >  2:142094/1‑139 (MQ=255)

                    gAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAgg                                                                                                            <  1:495911/139‑1 (MQ=255)

                     aGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           >  2:952723/1‑139 (MQ=255)

                     aGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           <  1:142094/139‑1 (MQ=255)

                                tGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACg                                                                                                <  1:952723/139‑1 (MQ=255)

                                            aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     >  1:1006577/1‑106 (MQ=255)

                                            aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     <  2:1006577/106‑1 (MQ=255)

                                            aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATGACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGa                                                                                    >  2:916801/1‑139 (MQ=255)

                                             aaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                   <  1:916801/139‑1 (MQ=255)

                                                  gcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                  <  1:120868/119‑1 (MQ=255)

                                                  gcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                  >  2:120868/1‑119 (MQ=255)

                                                   cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCa                                                                             >  1:1024799/1‑139 (MQ=255)

                                                     cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                           >  1:1121850/1‑139 (MQ=255)

                                                     cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                           <  2:1024799/139‑1 (MQ=255)

                                                              tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                  >  1:511301/1‑139 (MQ=255)

                                                                cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                >  1:706636/1‑139 (MQ=255)

                                                                          tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAg                                                                                                             <  2:306119/84‑1 (MQ=255)

                                                                          tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAg                                                                                                             >  1:306119/1‑84 (MQ=255)

                                                                           atGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                     <  2:706636/139‑1 (MQ=255)

                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAAtctc                                                                                                                      <  1:635232/71‑1 (MQ=255)

                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAAtctc                                                                                                                      >  2:635232/1‑71 (MQ=255)

                                                                               aaCATGCATTACAAAACTGTTTTAACTTTCTGACAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAcc                                                 >  2:621553/1‑139 (MQ=255)

                                                                                 cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     >  1:833637/1‑69 (MQ=255)

                                                                                 cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     <  2:833637/69‑1 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGtt                                                                                                                 <  2:1166019/71‑1 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGtt                                                                                                                 >  1:1166019/1‑71 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           >  1:33356/1‑77 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           <  2:33356/77‑1 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                             >  1:625362/1‑139 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                             >  1:653892/1‑139 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                             >  1:102336/1‑139 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                             >  2:374006/1‑139 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                             >  2:69161/1‑139 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           <  2:1071310/74‑1 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           >  1:1071310/1‑74 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                          >  2:1183930/1‑139 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                          >  1:825488/1‑139 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                          >  1:165258/1‑139 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                          >  1:677987/1‑139 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                          >  1:556053/1‑139 (MQ=255)

                                                                                        tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           <  2:1059934/72‑1 (MQ=255)

                                                                                        tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGgt                                                                                                           >  1:1059934/1‑72 (MQ=255)

                                                                                        tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                             >  2:791737/1‑86 (MQ=255)

                                                                                        tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                  <  2:327649/129‑1 (MQ=255)

                                                                                        tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                  >  1:327649/1‑129 (MQ=255)

                                                                                        tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                        >  1:288281/1‑139 (MQ=255)

                                                                                        tACAAAACTATTTTAACTTTCTGTCAACAGGTTTAGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                             <  1:791737/86‑1 (MQ=255)

                                                                                           aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCg                                     <  1:1183930/139‑1 (MQ=255)

                                                                                            aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                    >  1:192441/1‑139 (MQ=255)

                                                                                            aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                    <  2:653892/139‑1 (MQ=255)

                                                                                             aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCTAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                   >  1:363981/1‑91 (MQ=255)

                                                                                             aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                   <  2:363981/91‑1 (MQ=255)

                                                                                              aCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATg                                  <  1:301010/139‑1 (MQ=255)

                                                                                               cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAAtctc                                                                                                                      <  2:183542/54‑1 (MQ=255)

                                                                                               cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAAtctc                                                                                                                      >  1:183542/1‑54 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                   >  2:857177/1‑86 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                   <  1:857177/86‑1 (MQ=255)

                                                                                                    ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCa                            <  1:352043/139‑1 (MQ=255)

                                                                                                       aCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg                         <  1:1155518/139‑1 (MQ=255)

                                                                                                       aCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg                         <  1:69161/139‑1 (MQ=255)

                                                                                                          ttCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                        <  1:492996/73‑1 (MQ=255)

                                                                                                          ttCTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                        >  2:492996/1‑73 (MQ=255)

                                                                                                            cTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     <  2:488480/42‑1 (MQ=255)

                                                                                                            cTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTcg                                                                                                                     >  1:488480/1‑42 (MQ=255)

                                                                                                            cTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTTCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                    <  2:755124/123‑1 (MQ=255)

                                                                                                            cTGTCAACAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                    >  1:755124/1‑123 (MQ=255)

                                                                                                                cAACAGGTGTTGTAGTGGTCACT‑AAAATAACAATCTCGCGTTCCAGGTCGTCTCAGACGTTTCGTTCCTGAGCACGCAATCCCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCATTGTTGCTGaat                >  2:573986/1‑138 (MQ=255)

                                                                                                                 aaCAGGTTTTGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt               <  1:374006/139‑1 (MQ=255)

                                                                                                                        tttGTAATGGTCACTAAAAA‑AACAATCTCTCGTTCCAGGTCGTCCCAGACTTTTCGCTCCTGAGCATGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACGGAt        <  2:981511/139‑1 (MQ=255)

                                                                                                                        tttGTAATGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt        <  2:677987/139‑1 (MQ=255)

                                                                                                                             aaTGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt   <  2:192441/139‑1 (MQ=255)

                                                                                                                              aTGGTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCtt  <  2:288281/139‑1 (MQ=255)

                                                                                                                                ggTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                              >  1:152417/1‑109 (MQ=255)

                                                                                                                                ggTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                              <  2:152417/109‑1 (MQ=255)

                                                                                                                                ggTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt        <  2:766987/131‑1 (MQ=255)

                                                                                                                                ggTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt        >  1:766987/1‑131 (MQ=255)

                                                                                                                                ggTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc    <  1:204364/135‑1 (MQ=255)

                                                                                                                                ggTCACTAAAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc    >  2:204364/1‑135 (MQ=255)

CCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTT  >  NC_000913/4473998‑4474261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A