Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,138	(A)_6→7	coding (15/594 nt)	bdcR →	transcriptional repressor for divergent bdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,132	1	.	A	98.1%	350.9 / ‑1.5	107	coding (9/594 nt)	bdcR	transcriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (58/47); total (60/47)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 109 total aligned reads displayed.

ACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4474009‑4474264

aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTcg                                                                                                                        <  2:878602/139‑1 (MQ=255)

aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTcg                                                                                                                        >  2:1516094/1‑139 (MQ=255)

                       tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTcg                                                                                                                        <  1:1702308/116‑1 (MQ=255)

                       tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTcg                                                                                                                        >  2:1702308/1‑116 (MQ=255)

                                         ccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTcg                                                                                                                        <  2:1105295/98‑1 (MQ=255)

                                         ccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTcg                                                                                                                        >  1:1105295/1‑98 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCGGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                              <  1:783873/130‑1 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCGGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                              >  2:783873/1‑130 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              <  2:1816002/98‑1 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              >  1:1816002/1‑98 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:1051831/122‑1 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:1051831/1‑122 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  1:1796804/1‑139 (MQ=255)

                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  2:1548662/1‑139 (MQ=255)

                                                    ccTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCa                                                                    <  1:1516094/139‑1 (MQ=255)

                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              >  2:199170/1‑96 (MQ=255)

                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              <  1:199170/96‑1 (MQ=255)

                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   >  1:1273502/1‑139 (MQ=255)

                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑a                                                                                                                                       >  2:356843/1‑72 (MQ=255)

                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑a                                                                                                                                       <  1:356843/72‑1 (MQ=255)

                                                               tatataACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt                                                         >  1:1896593/1‑139 (MQ=255)

                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAAtctc                                                                                                                         <  2:46144/71‑1 (MQ=255)

                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGc                                                                                 >  2:558761/1‑111 (MQ=255)

                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGc                                                                                 <  1:558761/111‑1 (MQ=255)

                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  2:916226/1‑139 (MQ=255)

                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     <  1:916226/139‑1 (MQ=255)

                                                                     aCATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCa                                                   >  1:1244805/1‑139 (MQ=255)

                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              >  2:1363124/1‑79 (MQ=255)

                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              <  1:1363124/79‑1 (MQ=255)

                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        <  2:1891926/133‑1 (MQ=255)

                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        >  1:1891926/1‑133 (MQ=255)

                                                                       aTGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 <  2:1896593/139‑1 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAAtctc                                                                                                                         <  1:1891638/66‑1 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAAtctc                                                                                                                         >  2:1891638/1‑66 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTcg                                                                                                                        >  1:2031672/1‑67 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:2005413/1‑101 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:2005413/101‑1 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:276631/1‑139 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  2:1800016/1‑139 (MQ=255)

                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:1231558/1‑139 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAgg                                                                                                               >  2:868923/1‑73 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAgg                                                                                                               <  1:868923/73‑1 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                            <  1:515326/92‑1 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAg                                                                                   >  2:157391/1‑101 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAg                                                                                   <  1:157391/101‑1 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAtctc                                                                           >  1:1351385/1‑109 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAtctc                                                                           <  2:1351385/109‑1 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                             >  2:930863/1‑139 (MQ=255)

                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AAGAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                            >  2:515326/1‑92 (MQ=255)

                                                                            ttACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAAtctc                                                                                                                         >  2:1032720/1‑62 (MQ=255)

                                                                            ttACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAAtctc                                                                                                                         <  1:1032720/62‑1 (MQ=255)

                                                                             tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                           >  2:1988772/1‑139 (MQ=255)

                                                                               cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                         >  1:1877974/1‑139 (MQ=255)

                                                                                 aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              <  2:545903/68‑1 (MQ=255)

                                                                                 aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgt                                                                                                              >  1:545903/1‑68 (MQ=255)

                                                                                 aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                       <  1:1988772/139‑1 (MQ=255)

                                                                                  aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAAtctc                                                                                                                         >  2:1074572/1‑56 (MQ=255)

                                                                                  aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAAtctc                                                                                                                         <  1:1074572/56‑1 (MQ=255)

                                                                                    cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgtcg                                                                                                            >  2:432080/1‑67 (MQ=255)

                                                                                    cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGgtcg                                                                                                            <  1:432080/67‑1 (MQ=255)

                                                                                    cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGc                                    >  1:193355/1‑139 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:382701/86‑1 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:382701/1‑86 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:744680/1‑86 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      <  1:1557263/134‑1 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      >  2:902866/1‑134 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      >  2:1557263/1‑134 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      <  1:902866/134‑1 (MQ=255)

                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  2:1851625/1‑139 (MQ=255)

                                                                                         ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                >  2:757672/1‑74 (MQ=255)

                                                                                         ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                <  1:757672/74‑1 (MQ=255)

                                                                                          tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg                              <  2:1436550/139‑1 (MQ=255)

                                                                                            aCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg                            <  2:193355/139‑1 (MQ=255)

                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:869345/1‑76 (MQ=255)

                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:1064027/1‑76 (MQ=255)

                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:1064027/76‑1 (MQ=255)

                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:869345/76‑1 (MQ=255)

                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       >  2:252536/1‑139 (MQ=255)

                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       <  1:930863/139‑1 (MQ=255)

                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACT‑AAGAA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       >  2:801782/1‑139 (MQ=255)

                                                                                                  tGTCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                      <  2:1474607/139‑1 (MQ=255)

                                                                                                    tCAACAGGTTTTGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGaa                    <  2:1231558/139‑1 (MQ=255)

                                                                                                            ttttGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa            <  2:276631/139‑1 (MQ=255)

                                                                                                             tttGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  1:1851625/139‑1 (MQ=255)

                                                                                                              ttGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTc                                                       <  1:1266336/94‑1 (MQ=255)

                                                                                                              ttGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt          >  2:762251/1‑139 (MQ=255)

                                                                                                              ttGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt          >  2:1151221/1‑139 (MQ=255)

                                                                                                              atGTAATGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCTGCAAAAGTGCTTTTTc                                                       >  2:1266336/2‑94 (MQ=255)

                                                                                                                   aTGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  1:348213/58‑1 (MQ=255)

                                                                                                                   aTGGTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  2:348213/1‑58 (MQ=255)

                                                                                                                     ggTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCCATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGATTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  2:1936519/1‑139 (MQ=255)

                                                                                                                     ggTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 <  2:892318/109‑1 (MQ=255)

                                                                                                                     ggTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:976180/1‑109 (MQ=255)

                                                                                                                     ggTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  1:31838/1‑139 (MQ=255)

                                                                                                                     ggTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  1:532837/1‑139 (MQ=255)

                                                                                                                      gTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCg                                        <  2:577772/101‑1 (MQ=255)

                                                                                                                      gTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCg                                        >  1:577772/1‑101 (MQ=255)

                                                                                                                      gTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:1019043/1‑108 (MQ=255)

                                                                                                                      gTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 <  2:1019043/108‑1 (MQ=255)

                                                                                                                      gTCACTAAA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGt  >  1:501267/1‑139 (MQ=255)

ACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AA‑AA‑AACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4474009‑4474264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A