Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,138	(A)_6→7	coding (15/594 nt)	bdcR →	transcriptional repressor for divergent bdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,132	1	.	A	96.0%	236.9 / 1.6	75	coding (9/594 nt)	bdcR	transcriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (42/30); total (44/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4473998‑4474264

ccGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aaaa                                                                                                                                   >  2:1283167/1‑139 (MQ=255)

ccGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aaaa                                                                                                                                   >  1:639844/1‑139 (MQ=255)

     aCCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑aaaaaaaca                                                                                                                              <  2:639844/139‑4 (MQ=255)

               ccACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGtt                                                                                                                    >  1:644223/1‑139 (MQ=255)

                               cTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGAc                                                                                                    >  2:740767/1‑139 (MQ=255)

                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTc                                                                                                                   <  2:1273960/121‑1 (MQ=255)

                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTc                                                                                                                   >  1:1273960/1‑121 (MQ=255)

                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGtt                                                                                                 >  1:1585872/1‑139 (MQ=255)

                                  tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGtt                                                                                                 >  2:1594011/1‑139 (MQ=255)

                                       ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTGTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAATCAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                            >  2:1010126/1‑139 (MQ=255)

                                           tAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTg                                                                                        >  1:526381/1‑139 (MQ=255)

                                            aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGa                                                                                       >  1:1575600/1‑139 (MQ=255)

                                            aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGa                                                                                       >  2:862619/1‑139 (MQ=255)

                                             aaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  1:1010126/139‑1 (MQ=255)

                                             aaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:65355/139‑1 (MQ=255)

                                                   cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGgt                                                                                                              <  2:35476/109‑1 (MQ=255)

                                                   cccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGgt                                                                                                              >  1:35476/1‑109 (MQ=255)

                                                     cATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                              >  1:1544317/1‑139 (MQ=255)

                                                              tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  1:1016914/1‑139 (MQ=255)

                                                              tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  1:1112937/1‑139 (MQ=255)

                                                                cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   >  2:808957/1‑139 (MQ=255)

                                                                cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑a                                                                                                                                      <  2:1260874/72‑1 (MQ=255)

                                                                cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑a                                                                                                                                      >  1:1260874/1‑72 (MQ=255)

                                                                     tttATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTg                                                              >  2:1250262/1‑139 (MQ=255)

                                                                        attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtt                                                           >  1:1044937/1‑139 (MQ=255)

                                                                          tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt                                                         >  1:1299034/1‑139 (MQ=255)

                                                                           atGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        <  2:316467/139‑1 (MQ=255)

                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  2:1348511/1‑139 (MQ=255)

                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  1:406097/1‑139 (MQ=255)

                                                                              tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  2:101792/1‑139 (MQ=255)

                                                                               aaCATGCATTATAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAcc                                                    <  2:1044937/139‑1 (MQ=255)

                                                                                  aTGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 <  1:1283167/139‑1 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCCCT‑AAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                     <  2:337418/85‑1 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg                                                                                                                        >  2:1486349/1‑67 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTcg                                                                                                                        <  1:1486349/67‑1 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   >  2:1379064/1‑120 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   <  1:1379064/120‑1 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:585277/1‑139 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  2:777423/1‑139 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  2:1033614/1‑139 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                     >  1:337418/1‑85 (MQ=255)

                                                                                   tGCATTACAAAACTGTTTTAACTTTCTATCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:225588/1‑139 (MQ=255)

                                                                                      aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                             >  1:525754/1‑139 (MQ=255)

                                                                                         aCAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGttt                                          >  1:463076/1‑139 (MQ=255)

                                                                                          cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                         >  2:1229492/1‑139 (MQ=255)

                                                                                          cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                         <  2:198053/139‑1 (MQ=255)

                                                                                           aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAg                                                                                                                >  2:1186945/1‑67 (MQ=255)

                                                                                           aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAg                                                                                                                <  1:1186945/67‑1 (MQ=255)

                                                                                             aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAAtctc                                                                                                                         >  2:363074/1‑56 (MQ=255)

                                                                                             aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAAtctc                                                                                                                         <  1:363074/56‑1 (MQ=255)

                                                                                                 gTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa                                                  <  1:424026/123‑1 (MQ=255)

                                                                                                 gTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa                                                  >  2:424026/1‑123 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        >  2:1242604/1‑116 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        <  1:1242604/116‑1 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCt                                   <  2:43941/137‑1 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCt                                   >  1:43941/1‑137 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 <  1:1574625/139‑1 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  2:1289109/1‑139 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  2:1040118/1‑139 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:1504545/1‑139 (MQ=255)

                                                                                                  ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:474171/1‑139 (MQ=255)

                                                                                                     tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg                              <  1:1289109/139‑1 (MQ=255)

                                                                                                           tCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGc                        <  1:1250262/139‑1 (MQ=255)

                                                                                                            cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       >  1:120562/1‑139 (MQ=255)

                                                                                                             tatCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                      <  2:225588/137‑1 (MQ=255)

                                                                                                             tGTCAACAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                      <  2:1112937/139‑1 (MQ=255)

                                                                                                                 aaCAGGTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGt                  <  2:525754/139‑1 (MQ=255)

                                                                                                                      gTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 >  2:344500/1‑103 (MQ=255)

                                                                                                                      gTTTTGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 <  1:344500/103‑1 (MQ=255)

                                                                                                                       ttttGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGT‑TTGCTGAAGTTACTGAt           <  2:735164/139‑1 (MQ=255)

                                                                                                                        tttGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGATCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  1:1033614/139‑1 (MQ=255)

                                                                                                                        tttGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  2:463076/139‑1 (MQ=255)

                                                                                                                        tttGTAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  2:585277/139‑1 (MQ=255)

                                                                                                                            tAATGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc       <  1:101792/139‑1 (MQ=255)

                                                                                                                             aaTGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt      <  2:644223/139‑1 (MQ=255)

                                                                                                                              aTGGTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGTTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCtt     <  1:740767/139‑1 (MQ=255)

                                                                                                                                ggTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  2:1423322/1‑139 (MQ=255)

                                                                                                                                 gTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:941514/1‑93 (MQ=255)

                                                                                                                                 gTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                <  2:941514/93‑1 (MQ=255)

                                                                                                                                 gTCACTAAAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGt  >  1:1182081/1‑139 (MQ=255)

CCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAA‑CAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4473998‑4474264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A