Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,721,193	C→T	A414V (GCA→GTA)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,721,193	0	C	T	100.0%	130.5 / NA	41	A414V (GCA→GTA)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/22); total (19/22)

ATTCGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCG  >  NC_000913/2721064‑2721314

attcGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGAAGttt                                                                                                                  >  2:17373/1‑139 (MQ=255)

  tcGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTAt                                                                                                                >  2:899586/1‑139 (MQ=255)

     cccAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAAAGTGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCa                                                                                                             >  2:752584/1‑139 (MQ=255)

        aGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCg                                                                                                          >  1:938060/1‑139 (MQ=255)

             cgcTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGcc                                                                                                     <  1:924568/139‑1 (MQ=255)

                 gctCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCACAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGc                                                                                                 <  2:167029/139‑1 (MQ=255)

                      cGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCg                                                                                            >  2:224817/1‑139 (MQ=255)

                        cAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc                                                                                          <  1:224817/139‑1 (MQ=255)

                               aaGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGt                                                                                   <  1:899586/139‑1 (MQ=255)

                                      cAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCgg                                                                            <  2:902715/139‑1 (MQ=255)

                                        aGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaa                                                                          >  2:741757/1‑139 (MQ=255)

                                                  gAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa                                                                <  1:113598/139‑1 (MQ=255)

                                                   aaGCGGTAAAGCATCATTCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTGCTCGCAAGAGGTACGACGTTTATTCAGCGATGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCat                                                               <  1:650128/139‑2 (MQ=255)

                                                   aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCAc                                                               >  1:257654/1‑139 (MQ=255)

                                                    aGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGa                                                                                           <  1:527392/110‑1 (MQ=255)

                                                    aGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGa                                                                                           >  2:527392/1‑110 (MQ=255)

                                                         tAAAGCATCATCCCCGCAGC‑AATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt                                                        <  1:220594/139‑1 (MQ=255)

                                                            aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTAt                                                      <  2:938060/139‑1 (MQ=255)

                                                            aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTAt                                                      <  2:275728/139‑1 (MQ=255)

                                                              catcatCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGc                                                    <  1:1019281/139‑1 (MQ=255)

                                                                  atcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTa                                                                                       <  1:926388/100‑1 (MQ=255)

                                                                  atcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTa                                                                                       >  2:926388/1‑100 (MQ=255)

                                                                  atcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCatat                                                <  1:741757/139‑1 (MQ=255)

                                                                   tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGTACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATAtg                                               >  2:162104/1‑139 (MQ=255)

                                                                   tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg                                                                                    >  2:180117/1‑102 (MQ=255)

                                                                   tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg                                                                                    <  1:180117/102‑1 (MQ=255)

                                                                   tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTa                                                       <  1:736097/131‑1 (MQ=255)

                                                                   tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTa                                                       >  2:736097/1‑131 (MQ=255)

                                                                               atatGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGTAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCgg                                   >  2:876833/1‑139 (MQ=255)

                                                                                 atGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCg                                 <  1:803731/139‑1 (MQ=255)

                                                                                          tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCt                                                           <  2:19176/104‑1 (MQ=255)

                                                                                          tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCt                                                           >  1:19176/1‑104 (MQ=255)

                                                                                               aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg                                                             <  2:960304/97‑1 (MQ=255)

                                                                                               aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg                                                             >  1:960304/1‑97 (MQ=255)

                                                                                                cGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt                  >  2:39195/1‑139 (MQ=255)

                                                                                                    cTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCatat                                                >  1:396711/1‑105 (MQ=255)

                                                                                                    cTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCatat                                                <  2:396711/105‑1 (MQ=255)

                                                                                                               aGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGc   >  2:696548/1‑139 (MQ=255)

                                                                                                                gCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCg  <  1:936121/139‑1 (MQ=255)

                                                                                                                         gCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc                                                                                          <  1:927427/42‑1 (MQ=255)

                                                                                                                         gCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc                                                                                          >  2:927427/1‑42 (MQ=255)

ATTCGCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCG  >  NC_000913/2721064‑2721314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A