Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,721,193 |
C→T |
A414V (GCA→GTA) |
pka → |
protein lysine acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,721,193 | 0 | C | T | 98.2%
| 182.1
/ ‑6.8
| 57 | A414V (GCA→GTA) | pka | protein lysine acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (26/30); minor base A (0/1); total (26/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCC > NC_000913/2721068‑2721320
|
gCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCGGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCCCAAGAGGTACGACGTTTATTc > 2:1012137/1‑139 (MQ=255)
cgcTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGcc < 1:1708662/139‑1 (MQ=255)
ctgctCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCgg > 2:1461802/1‑139 (MQ=255)
gctCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGc < 1:440167/139‑1 (MQ=255)
tCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTg < 1:1012137/139‑1 (MQ=255)
cccGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc > 2:191704/1‑139 (MQ=255)
cccGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc > 2:1308217/1‑139 (MQ=255)
aGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTAc > 1:718029/1‑139 (MQ=255)
aTTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaagga < 2:1166730/139‑1 (MQ=255)
aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc > 2:863813/1‑112 (MQ=255)
aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc < 1:863813/112‑1 (MQ=255)
aGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGTACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACt > 2:1013831/1‑139 (MQ=255)
gCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg > 1:1008849/1‑139 (MQ=255)
gTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa > 2:965025/1‑133 (MQ=255)
gTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa < 1:965025/133‑1 (MQ=255)
aaaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGTACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt < 1:1013831/139‑1 (MQ=255)
aaaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt < 1:328097/139‑1 (MQ=255)
aaaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCAGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCtttt < 1:547520/139‑1 (MQ=255)
aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGGGGCGAGCACTCCTCGCAAGAGGAACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTCTTAt < 2:1008849/139‑1 (MQ=255)
aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTAt < 1:1308217/139‑1 (MQ=255)
tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAAc > 2:1298399/1‑117 (MQ=255)
tcCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAAc < 1:1298399/117‑1 (MQ=255)
gcagcaAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTac > 2:333630/1‑60 (MQ=255)
gcagcaAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTac < 1:333630/60‑1 (MQ=255)
caAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTAc < 1:1461802/139‑1 (MQ=255)
caAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTAc < 2:718029/139‑1 (MQ=255)
aaTATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCgg < 1:480271/99‑1 (MQ=255)
aaTATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCgg > 2:480271/1‑99 (MQ=255)
tctTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATc > 1:1355647/1‑139 (MQ=255)
ttGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg > 2:272023/1‑103 (MQ=255)
ttGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg < 1:272023/103‑1 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc > 2:898009/1‑69 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGc < 1:898009/69‑1 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCg < 1:1413943/71‑1 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCg > 2:1413943/1‑71 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc > 2:417740/1‑73 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGAc < 1:417740/73‑1 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg < 1:11300/102‑1 (MQ=255)
tGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTg > 2:11300/1‑102 (MQ=255)
gACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa < 1:1462205/95‑1 (MQ=255)
gACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCa > 2:1462205/1‑95 (MQ=255)
gACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCaa > 2:1647197/1‑139 (MQ=255)
aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTg > 2:432023/1‑63 (MQ=255)
aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTg < 1:432023/63‑1 (MQ=255)
aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg < 1:1679868/74‑1 (MQ=255)
aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCg > 2:1679868/1‑74 (MQ=255)
cGAGCTGGTGCGGCGAGCACTCCTCGCAAGAGGTACGACGTTTACTCAGCGAAGCCGGGCTGGCGACCTACCGTACCCCGGAAGGAACCATCACTGGTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt < 2:689216/139‑1 (MQ=255)
cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCAt > 1:167089/1‑78 (MQ=255)
cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCAt < 2:167089/78‑1 (MQ=255)
cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt > 2:1299374/1‑126 (MQ=255)
cgAGCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACt < 1:1299374/126‑1 (MQ=255)
gCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCg < 2:1355647/139‑1 (MQ=255)
gCACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCg < 1:1647197/139‑1 (MQ=255)
cACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaa < 1:1191522/66‑1 (MQ=255)
cACTCCTCGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCggaa > 2:1191522/1‑66 (MQ=255)
ctcGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGccc > 2:1427888/1‑139 (MQ=255)
ctcGCAAGAGGTACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGccc < 1:191704/139‑1 (MQ=255)
|
GCCCAGCGCCGCTGCTCCCGCAACAGAAAGCGCGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCC > NC_000913/2721068‑2721320
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
