Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I2 R1 762 35.5 1545288 95.5% 1475750 117.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 883,665 IS1 (–) +9 bp [mdfA] [mdfA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 279930NA (NA)28 (0.780) 23/228 0.5 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 883665 = 0 (0.000)intergenic (‑277/‑8) ybjG/mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein
* ? NC_000913 = 8836730 (0.000)47 (1.290) 37/232 0.1 100% coding (1/1233 nt) mdfA multidrug efflux system protein
?NC_000913 = 1050545 NA (NA)noncoding (768/768 nt) IS1 repeat region

TGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCga‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/279793‑279930
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGT  >  NC_000913/883665‑883803
                                                                                                                                                                                                                                                                                     
TGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCG                                                                                                                                            <  1:410122/139‑1
                                CACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTG                                                                                                            <  2:666786/139‑1
                                             GCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGTAGCACCTCAAAAACACCATCATACATTAAATGAGTAAGTTGGCAGCATCACCGAAATTCCATGCAAAATAAATTAGCTTCCGGTGCC                                                                                                          >  1:469132/1‑128
                                             GCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACG                                                                                               >  1:638728/1‑139
                                             GCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCC                                                                                                          <  2:469132/128‑1
                                              CTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGT                                                                                              <  1:288469/139‑1
                                                           ACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCG                                                                                        <  2:664643/132‑1
                                                           ACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCG                                                                                        >  1:664643/1‑132
                                                           ACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTC                                                                                                                 <  1:352741/107‑1
                                                           ACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTC                                                                                                                 >  2:352741/1‑107
                                                                    AGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCT                                                                        >  2:55640/1‑139
                                                                         CAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTG                                                                   >  2:420076/1‑139
                                                                          AGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGG                                                                  >  1:347810/1‑139
                                                                          AGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTATCTGTCTGG                                                                  >  2:529516/1‑139
                                                                                          CCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATG                                                                                                                                  <  2:319520/59‑1
                                                                                          CCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATG                                                                                                                                  >  1:319520/1‑59
                                                                                                         CATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAA                                   <  1:317537/139‑1
                                                                                                                TAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGG                                      <  2:503214/129‑1
                                                                                                                TAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGG                                      >  1:503214/1‑129
                                                                                                                   ATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATT                         >  2:281038/1‑139
                                                                                                                   ATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAA                                   <  2:114088/129‑1
                                                                                                                   ATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAA                                   >  1:114088/1‑129
                                                                                                                              GGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTC                                                                                             >  2:516356/1‑60
                                                                                                                              GGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTC                                                                                             <  1:516356/60‑1
                                                                                                                                CAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGT            >  1:374809/1‑139
                                                                                                                                    ATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCT                                                                          <  2:130785/73‑1
                                                                                                                                    ATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCT                                                                          >  1:130785/1‑73
                                                                                                                                      CACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTT                                                   >  2:37271/1‑94
                                                                                                                                      CACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTT                                                   <  1:37271/94‑1
                                                                                                                                          GAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGT  <  2:374809/139‑1
                                                                                                                                                                                                                                                                                     
TGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCga‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/279793‑279930
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGT  >  NC_000913/883665‑883803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A