Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A8 F1 I2 R1 | 765 | 53.2 | 2691310 | 93.4% | 2513683 | 103.8 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC JC | NC_000913 | 883,665 | IS1 (–) +9 bp | [mdfA] | [mdfA] | |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 19796 = | NA (NA) | 71 (1.310) | 43/206 | 0.2 | 100% | noncoding (768/768 nt) | IS1 | repeat region |
| ? | NC_000913 | = 883673 | 0 (0.000) | coding (1/1233 nt) | mdfA | multidrug efflux system protein | |||||
| * | ? | NC_000913 | = 258675 | NA (NA) | 53 (0.970) | 40/206 | 0.2 | 100% | noncoding (1/768 nt) | IS1 | repeat region |
| ? | NC_000913 | 883665 = | 0 (0.000) | intergenic (‑277/‑8) | ybjG/mdfA | undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein | |||||
CATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/19933‑19796‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTACATTATTACGAGTATAGCTACGC < NC_000913/883673‑883551 CATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCT < 2:19373/139‑1 AATCTGATGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTACATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCT > 2:881758/1‑139 CTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCG < 1:326330/139‑1 AGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCG > 2:476282/1‑136 AGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCG < 1:476282/136‑1 TGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCG > 1:75054/1‑139 GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATGAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG < 1:557242/139‑1 GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG < 2:135630/139‑1 GCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTACATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG < 1:881758/139‑1 ACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCA < 2:1097529/139‑1 GGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAATATAAAGCACTATCAATAAGTTGGAGTCATTACCTGCAATTTCT < 1:899710/108‑1 GGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCT > 2:899710/1‑108 AAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAA > 2:1156079/1‑139 CTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGC > 1:546636/1‑95 CTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGC < 2:546636/95‑1 CTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAA > 2:1138660/1‑86 CTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAA < 1:1138660/86‑1 TGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCG < 1:601358/74‑1 TGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCG > 2:601358/1‑74 TCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG < 1:462699/81‑1 TCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG > 2:462699/1‑81 CGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCA > 1:1310078/1‑79 CGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCA < 2:1310078/79‑1 CGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCG > 2:1339535/1‑62 CGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCG < 1:1339535/62‑1 GGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCAAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCA < 1:969018/139‑1 AACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAG < 1:683137/69‑1 AACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAG > 2:683137/1‑69 AACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATA > 1:254061/1‑58 AACATAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATA < 2:254061/58‑1 TAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTGTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGCATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTACATTATT < 1:900893/139‑1 TAAAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTACATTATT < 2:75054/139‑1 AAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCT < 2:159767/105‑1 AAACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCT > 1:159767/1‑105 AACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG < 2:432416/57‑1 AACACTATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG > 1:432416/1‑57 ATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCA < 2:2526/105‑1 ATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCA > 1:2526/1‑105 ATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTT < 1:1265017/59‑1 ATCAATAAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTT > 2:1265017/1‑59 AAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGC < 2:22612/79‑1 AAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGC > 1:22612/1‑79 AAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG > 2:1028267/1‑45 AAGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCG < 1:1028267/45‑1 AGTTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTACATTATTACGATTATAGCTACGC > 2:456483/1‑139 TTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTAC > 2:609780/1‑115 TTGGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTAC < 1:609780/115‑1 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTACATT < 1:1007826/116‑1 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTACATT > 2:1007826/1‑116 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGC > 1:797478/1‑103 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGC < 2:797478/103‑1 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTG > 2:721069/1‑82 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTG < 1:721069/82‑1 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGC > 2:456236/1‑41 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGC < 1:456236/41‑1 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAA < 2:1257444/35‑1 GGAGTCATTACCTGCAATTTCTTCGCCAATAATAA > 1:1257444/1‑35 AGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGC > 2:1007293/1‑101 AGTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGC < 1:1007293/101‑1 GTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAG > 2:660709/1‑40 GTCATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAG < 1:660709/40‑1 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCA < 2:656573/98‑1 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCA > 1:656573/1‑98 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAAC > 2:256810/1‑86 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAAC < 1:256810/86‑1 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAG > 1:923124/1‑50 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAG < 2:923124/50‑1 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTT < 2:461269/42‑1 ATTACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTT > 1:461269/1‑42 TACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCA < 1:74060/86‑1 TACCTGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCA > 2:74060/1‑86 CATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/19933‑19796‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGCAATTTCTTCGCCAATAATAATCGCGCAGAGTTTAATAAAAGCGCAGCTAACGAGAAAGCGAATTTTGTAGCTGAAACCACGGTTAAGCACATTCTTACATTATTACGAGTATAGCTACGC < NC_000913/883673‑883551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |