Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I2 R1 757 38.1 1607544 96.5% 1551279 117.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 883,665 IS1 (–) +9 bp [mdfA] [mdfA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 258675NA (NA)46 (1.190) 35/232 0.1 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 883665 = 0 (0.000)intergenic (‑277/‑8) ybjG/mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein
* ? NC_000913 = 8836730 (0.000)52 (1.350) 36/232 0.1 100% coding (1/1233 nt) mdfA multidrug efflux system protein
?NC_000913 = 1050545 NA (NA)noncoding (768/768 nt) IS1 repeat region

CCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/258544‑258675
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGG  >  NC_000913/883665‑883802
                                                                                                                                                                                                                                                                              
CCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAAAAgcg                                                                                                                                     <  2:650978/139‑4
   GCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAAAAgcgcca                                                                                                                                  <  2:341928/139‑7
      GTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCA                                                                                                                               <  1:194421/139‑1
         CTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAAAAgcgccatctaac                                                                                                                            >  1:781104/1‑127
                   TACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCT                                                                                                                  >  1:271155/1‑139
                    ACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTT                                                                                                                 <  1:126932/139‑1
                            CCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAAAAgcgc                                                                                                                                    >  2:260235/1‑108
                            CCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCAAAAgcgc                                                                                                                                    <  1:260235/112‑5
                               CGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGG                                                                                                      >  1:90461/1‑139
                                    GTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGG                                                                                                 >  1:278184/1‑139
                                                GAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTT                                                                                     >  1:38950/1‑139
                                                 AGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTA                                                                                    >  1:139765/1‑139
                                                       AGCTGATGTAAACAGACGCCACGGGAGCACCTCAAAAACACCCTCATACATTAAATCAGTAAGTTGTCAGCATCACCGAAACTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTC                                                                              <  1:412314/139‑1
                                                           GATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTC                                                                          <  2:139765/139‑1
                                                                   CAGAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTG                                                                  >  1:48993/1‑139
                                                                     GAAGCCACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGT                                                                <  2:48993/139‑1
                                                                           ACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGC                                                                                                                                    >  2:556366/1‑65
                                                                           ACTGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGC                                                                                                                                    <  1:556366/65‑1
                                                                             TGGAGCACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACG                                                        >  2:58624/1‑139
                                                                                  CACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTT                                                                               <  1:736985/111‑1
                                                                                  CACCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTT                                                                               >  2:736985/1‑111
                                                                                    CCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTC                                                 >  1:65412/1‑139
                                                                                    CCTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTC                                                 >  2:370649/1‑139
                                                                                     CTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGC                                                                                                          <  1:289831/81‑1
                                                                                     CTCAAAAACACCATCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGC                                                                                                          >  2:289831/1‑81
                                                                                                  TCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCA                                   >  1:301769/1‑139
                                                                                                  TCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCA                                   >  2:660865/1‑139
                                                                                                  TCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGC                                    >  1:519647/1‑138
                                                                                                  TCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGC                                    <  2:519647/138‑1
                                                                                                  TCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAA                                                                                                                             <  1:113206/49‑1
                                                                                                  TCATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAA                                                                                                                             >  2:113206/1‑49
                                                                                                   CATACATTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAA                                  <  2:90461/139‑1
                                                                                                        atTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTT                                                  <  2:353284/116‑1
                                                                                                        atTAAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTT                                                  >  1:353284/3‑118
                                                                                                           AAATCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGA                          >  2:652317/1‑139
                                                                                                            AATCAGTAAGTTGGCAGCATCACTGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTC                                                                              <  2:477766/86‑1
                                                                                                            AATCAGTAAGTTGGCAGCATCACCGAAATTGCGTGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTC                                                                              >  1:477766/1‑86
                                                                                                              TCAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTC                       <  2:145396/139‑1
                                                                                                               CAGTAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCA                      <  1:422695/139‑1
                                                                                                                  TAAGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACC                   >  1:439684/1‑139
                                                                                                                    AGTTGGCAGCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCG                 <  1:652317/139‑1
                                                                                                                            GCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCG                                                                                       >  2:111594/1‑61
                                                                                                                            GCATCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCG                                                                                       <  1:111594/61‑1
                                                                                                                               TCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAC                                                                                   >  2:301132/1‑62
                                                                                                                               TCACCGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAC                                                                                   <  1:301132/62‑1
                                                                                                                                   CGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGG  <  2:439684/139‑1
                                                                                                                                                                                                                                                                              
CCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/258544‑258675
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGG  >  NC_000913/883665‑883802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A