Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
757 |
38.1 |
1607544 |
96.5% |
1551279 |
117.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,821,867 |
(A)8→7 |
intergenic (‑248/+51) |
chbB ← / ← osmE |
N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,821,860 | 0 | A | . | 100.0%
| 243.9
/ NA
| 57 | intergenic (‑241/+58) | chbB/osmE | N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (34/23); total (34/23) |
ACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA > NC_000913/1821738‑1821990
|
aCGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAAt > 2:658961/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAACTTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 1:136063/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:779349/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:105936/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:124982/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 1:715592/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:142417/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:208168/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:33765/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 1:435812/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:552989/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:605205/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgt < 1:628420/138‑1 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgt > 2:628420/1‑138 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGACAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGtt > 2:618669/1‑133 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGACAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGtt < 1:618669/133‑1 (MQ=255)
tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAc > 2:373963/1‑139 (MQ=255)
gTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc < 1:64139/139‑1 (MQ=255)
tCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt > 1:388894/1‑139 (MQ=255)
ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt > 2:463487/1‑139 (MQ=255)
taAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt < 1:762590/127‑1 (MQ=255)
taAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt > 2:762590/1‑127 (MQ=255)
tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGgc > 2:147291/1‑87 (MQ=255)
tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGgc < 1:147291/87‑1 (MQ=255)
ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg < 2:435812/139‑1 (MQ=255)
ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg < 1:124982/139‑1 (MQ=255)
ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa < 1:552989/139‑1 (MQ=255)
ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATc > 2:684897/1‑139 (MQ=255)
gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa < 1:33765/139‑1 (MQ=255)
gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa < 1:142417/139‑1 (MQ=255)
aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg > 2:141287/1‑139 (MQ=255)
gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc < 1:684897/139‑1 (MQ=255)
ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCGAGCTTTCAGACGAATTGATTACTTCCCAGCCTGTGGATCAGTGTCGTa > 1:27811/1‑139 (MQ=255)
tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg < 2:555205/115‑1 (MQ=255)
tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg > 1:555205/1‑115 (MQ=255)
ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc > 2:146761/1‑139 (MQ=255)
ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc > 2:102686/1‑139 (MQ=255)
ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc > 1:328614/1‑139 (MQ=255)
acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCTGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca < 2:388894/139‑1 (MQ=255)
acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca < 2:328614/139‑1 (MQ=255)
acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca < 1:652121/139‑1 (MQ=255)
caTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcac > 2:524010/1‑139 (MQ=255)
tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAGTCa > 1:512863/1‑81 (MQ=255)
tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCa < 2:512863/81‑1 (MQ=255)
ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc > 1:542539/1‑79 (MQ=255)
ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc < 2:542539/79‑1 (MQ=255)
cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTc > 2:266782/1‑139 (MQ=255)
gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg < 2:781884/103‑1 (MQ=255)
gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg > 1:781884/1‑103 (MQ=255)
ggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTg < 1:105936/139‑1 (MQ=255)
gCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGa < 1:141287/139‑1 (MQ=255)
tAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc < 1:266782/139‑1 (MQ=255)
aGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCg < 2:715592/139‑1 (MQ=255)
aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa > 2:385933/1‑139 (MQ=255)
aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa > 1:99968/1‑139 (MQ=255)
aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa > 1:153652/1‑72 (MQ=255)
aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa < 2:153652/72‑1 (MQ=255)
|
ACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTA > NC_000913/1821738‑1821990
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
