Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,821,867	(A)_8→7	intergenic (‑248/+51)	chbB ← / ← osmE	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,821,860	0	A	.	97.4%	304.7 / 1.9	77	intergenic (‑241/+58)	chbB/osmE	N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (43/32); total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

ACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821747‑1821994

aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGcc                                                                                                                   >  2:1143960/1‑134 (MQ=255)

aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGcc                                                                                                                   <  1:1143960/134‑1 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCgt                                                                                                          >  1:132705/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATgg                                                                                                                     <  1:409677/127‑1 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATgg                                                                                                                     >  2:409677/1‑127 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:810192/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:112611/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:1087420/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:57631/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:244138/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:512064/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:357126/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:406587/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:490566/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:263771/1‑139 (MQ=255)

     aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑AAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        >  2:1197469/1‑139 (MQ=255)

          tAGTATTTCTTTGACCTTAT‑AATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACt                                                                                                   >  2:165053/1‑139 (MQ=255)

             tATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCa                                                                                                 <  2:406587/139‑1 (MQ=255)

                  cTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCtt                                                                                            >  2:255254/1‑139 (MQ=255)

                      gACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                        >  2:239873/1‑139 (MQ=255)

                        ccTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  1:244138/139‑1 (MQ=255)

                                    tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                                  <  2:764444/115‑1 (MQ=255)

                                    tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                                  >  1:764444/1‑115 (MQ=255)

                                      tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  1:941600/1‑139 (MQ=255)

                                       ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:263771/139‑1 (MQ=255)

                                         ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:255254/139‑1 (MQ=255)

                                         ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:357126/139‑1 (MQ=255)

                                          cTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:132705/139‑1 (MQ=255)

                                            tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaacc                                                                                                                                >  2:402312/1‑76 (MQ=255)

                                            tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaacc                                                                                                                                <  1:402312/78‑3 (MQ=255)

                                            tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGcc                                                                  >  2:236035/1‑139 (MQ=255)

                                              aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  2:512064/139‑1 (MQ=255)

                                                  gtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGa                                                            >  1:580496/1‑139 (MQ=255)

                                                   tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  1:112611/139‑1 (MQ=255)

                                                    ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGcc                                                                  >  2:1189313/1‑131 (MQ=255)

                                                    ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGcc                                                                  <  1:1189313/131‑1 (MQ=255)

                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             <  2:221275/84‑1 (MQ=255)

                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             >  1:221275/1‑84 (MQ=255)

                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  2:733057/1‑139 (MQ=255)

                                                        aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  1:130474/1‑139 (MQ=255)

                                                         tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:1215830/123‑1 (MQ=255)

                                                         tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     >  1:1215830/1‑123 (MQ=255)

                                                          gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:1087420/139‑1 (MQ=255)

                                                          gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  2:580496/139‑1 (MQ=255)

                                                             ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:905180/1‑139 (MQ=255)

                                                               tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               >  2:292491/1‑139 (MQ=255)

                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCttt                                                                                           <  1:216353/91‑1 (MQ=255)

                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCttt                                                                                           >  2:216353/1‑91 (MQ=255)

                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:280817/113‑1 (MQ=255)

                                                                   cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     >  2:280817/1‑113 (MQ=255)

                                                                    acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:341313/139‑1 (MQ=255)

                                                                           gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                   >  1:8790/1‑139 (MQ=255)

                                                                                   aatTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCg                           <  2:57631/139‑1 (MQ=255)

                                                                                     ttCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTTTGATAACCGGa                         <  2:905180/139‑1 (MQ=255)

                                                                                      tCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAg                        <  1:503694/139‑1 (MQ=255)

                                                                                       cgcgAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGt                       >  2:81998/1‑139 (MQ=255)

                                                                                             gATAATTAAGAACTCAGGCC‑‑AAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACTAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:1197469/139‑1 (MQ=255)

                                                                                              aTAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:490566/139‑1 (MQ=255)

                                                                                              aTAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:292491/139‑1 (MQ=255)

                                                                                              aTAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:239873/139‑1 (MQ=255)

                                                                                                   tAAGAACTCAGGCC‑AAAAAAACCTGCGCAATGGCCGGTTTCCGTTGTTACTCAATCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  1:802637/139‑1 (MQ=255)

                                                                                                   tAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:941600/139‑1 (MQ=255)

                                                                                                       aaCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGt       >  2:1186354/1‑139 (MQ=255)

                                                                                                        aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                             <  2:839946/68‑1 (MQ=255)

                                                                                                        aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                             >  1:839946/1‑68 (MQ=255)

                                                                                                        aCTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  1:1152541/1‑139 (MQ=255)

                                                                                                         cTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:1030200/92‑1 (MQ=255)

                                                                                                         cTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  2:1030200/1‑92 (MQ=255)

                                                                                                         cTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTat     <  2:130474/139‑1 (MQ=255)

                                                                                                          tCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatc    >  2:1164351/1‑139 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      >  2:503715/1‑55 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  1:503715/55‑1 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAg                        <  2:429080/117‑1 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAg                        >  1:429080/1‑117 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:633959/1‑139 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  1:514608/1‑139 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  1:521372/1‑139 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:126622/1‑139 (MQ=255)

                                                                                                            aGGCC‑AAAAAAACCGGCGAAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:802637/1‑139 (MQ=255)

ACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821747‑1821994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A