Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,821,867 |
(A)8→7 |
intergenic (‑248/+51) |
chbB ← / ← osmE |
N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,821,860 | 0 | A | . | 100.0%
| 129.4
/ NA
| 30 | intergenic (‑241/+58) | chbB/osmE | N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (16/14); total (16/14) |
ATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAC > NC_000913/1821743‑1821980
|
ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCg > 2:416548/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTGACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGGGCAATGGCCGGTTTCCgtt > 2:549236/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaaccg > 1:111376/1‑115 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaaccg < 2:111376/118‑4 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 2:428637/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 1:107049/1‑139 (MQ=255)
aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCgtt > 1:319478/1‑139 (MQ=255)
tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAc > 1:69998/1‑139 (MQ=255)
tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTAc > 2:355692/1‑139 (MQ=255)
tataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa > 1:307556/1‑139 (MQ=255)
tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc > 1:200332/1‑139 (MQ=255)
ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg < 1:428637/139‑1 (MQ=255)
ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa < 1:377620/139‑1 (MQ=255)
aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg < 2:319478/139‑1 (MQ=255)
tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc < 1:364470/89‑1 (MQ=255)
tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTc > 2:364470/1‑89 (MQ=255)
gACCCTCTTCACATTTCGGGCAAGTAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCTGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc < 1:392315/139‑1 (MQ=255)
gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc < 1:355692/139‑1 (MQ=255)
gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc < 1:342933/139‑1 (MQ=255)
ctctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa < 1:289494/118‑1 (MQ=255)
ctctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa > 2:289494/1‑118 (MQ=255)
ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt > 1:772676/1‑126 (MQ=255)
ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt < 2:772676/126‑1 (MQ=255)
cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc > 2:823812/1‑123 (MQ=255)
cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc < 1:823812/123‑1 (MQ=255)
gCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGa > 2:588035/1‑139 (MQ=255)
aatTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAAGTCTGATAACCg < 2:69998/139‑1 (MQ=255)
aaGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt > 2:694249/1‑111 (MQ=255)
aaGATAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt < 1:694249/111‑1 (MQ=255)
aTAATTAAGAACTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc < 2:644524/139‑1 (MQ=255)
cTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca < 2:430591/102‑1 (MQ=255)
cTCAGGCC‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca > 1:430591/1‑102 (MQ=255)
|
ATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAC > NC_000913/1821743‑1821980
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
