Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A36 F79 I1 R1 25 416.7 19229882 94.6% 18191468 142.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,209,475 A→T N387I (AAC→ATC)  PP_1948 → benzaldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,209,4750AT98.9% 1326.1 / 0.5 373N387I (AAC→ATC) PP_1948benzaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (206/163);  total (208/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 376 total aligned reads displayed.

GCCCCCTAACGACCAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCAACAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGACCACCGATCTGGCTCGCG  >  NC_002947/2209328‑2209621
                                                                                                                                                   |                                                                                                                                                  
     cTAACGACCAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCg                                                                                                                                             >  1:9580712/1‑150 (MQ=255)
       aaCGACCAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGAt                                                                                                                                           >  1:5771949/1‑150 (MQ=255)
       aaCGACCAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGAt                                                                                                                                           >  1:9142769/1‑150 (MQ=255)
       aaCGACCAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGAt                                                                                                                                           <  2:2897169/150‑1 (MQ=255)
          gACCAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGACTACACCAACGTCATCAACTCGATGCg                                                                                                                                        >  1:3680629/1‑150 (MQ=255)
            ccAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGa                                                                                                                                      >  2:7161442/1‑150 (MQ=255)
             cAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGAt                                                                                                                                     <  1:6580371/150‑1 (MQ=255)
             cAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGAt                                                                                                                                     >  2:7781980/1‑150 (MQ=255)
             cAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGAt                                                                                                                                     >  1:2136806/1‑150 (MQ=255)
               aGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTg                                                                                                                                   <  2:5268767/150‑1 (MQ=255)
                  cTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTc                                                                                                                                <  2:1379635/150‑1 (MQ=255)
                   tCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCg                                                                                                                               >  1:6929196/1‑150 (MQ=255)
                   tCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCg                                                                                                                               >  1:8372474/1‑150 (MQ=255)
                   tCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCg                                                                                                                               <  2:7361395/150‑1 (MQ=255)
                   tCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCg                                                                                                                               >  1:4018736/1‑150 (MQ=255)
                   tCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCg                                                                                                                               >  2:8576191/1‑150 (MQ=255)
                                gTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTc                                                                                                                  <  2:2195773/150‑1 (MQ=255)
                                gTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTc                                                                                                                  <  2:5080472/150‑1 (MQ=255)
                                gTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTc                                                                                                                  >  1:1053316/1‑150 (MQ=255)
                                gTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTc                                                                                                                  <  1:1853308/150‑1 (MQ=255)
                                gTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTc                                                                                                                  <  2:1782293/150‑1 (MQ=255)
                                gTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCGTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTc                                                                                                                  <  2:6399067/150‑1 (MQ=255)
                                 ttCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCg                                                                                                                 <  1:5513815/150‑1 (MQ=255)
                                   cATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGa                                                                                                               <  2:344240/150‑1 (MQ=255)
                                            aaCGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTg                                                                                                      >  1:3508041/1‑150 (MQ=255)
                                            aaCGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTg                                                                                                      >  1:4893067/1‑150 (MQ=255)
                                            aaCGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTg                                                                                                      >  1:6337884/1‑150 (MQ=255)
                                               gAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTa                                                                                                   >  1:4947107/1‑150 (MQ=255)
                                                aGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTAc                                                                                                  <  2:2275062/150‑1 (MQ=255)
                                                   tGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGa                                                                                               >  2:8997973/1‑150 (MQ=255)
                                                   tGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGGGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGa                                                                                                               >  2:5826305/1‑134 (MQ=255)
                                                       cATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTcc                                                                                           >  1:1209998/1‑150 (MQ=255)
                                                       cATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTcc                                                                                           >  1:7282096/1‑150 (MQ=255)
                                                       cATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTcc                                                                                           <  1:7997962/150‑1 (MQ=255)
                                                       cATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTcc                                                                                           >  2:611561/1‑150 (MQ=255)
                                                       cATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTcc                                                                                           >  1:6062662/1‑150 (MQ=255)
                                                       cATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCAACAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTcc                                                                                           <  1:7454743/150‑1 (MQ=255)
                                                        aTCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCg                                                                                                                               <  1:3310940/113‑1 (MQ=255)
                                                          ccAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGtt                                                                                        <  1:4796572/150‑1 (MQ=255)
                                                           cAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTc                                                                                       >  1:2543012/1‑150 (MQ=255)
                                                                gggggCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGa                                                                                  <  1:5198813/150‑1 (MQ=255)
                                                                  gggCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACg                                                                                >  2:6232608/1‑150 (MQ=255)
                                                                   ggCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGa                                                                               <  2:8294323/150‑1 (MQ=255)
                                                                    gCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGAccc                                                                                                            >  1:6253499/1‑120 (MQ=255)
                                                                    gCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGaa                                                                              <  2:7004787/150‑1 (MQ=255)
                                                                        aTTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACg                                                                          >  2:6547444/1‑150 (MQ=255)
                                                                         ttGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAg                                                                                    >  1:772226/1‑139 (MQ=255)
                                                                         ttGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAg                                                                                    <  2:772226/139‑1 (MQ=255)
                                                                          tGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAg                                                                        <  1:8612427/150‑1 (MQ=255)
                                                                            cTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGc                                                                      >  2:4436904/1‑150 (MQ=255)
                                                                             ttGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGa                                                                                                                                            <  2:2000846/79‑1 (MQ=255)
                                                                             ttGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCg                                                                     >  1:3071533/1‑150 (MQ=255)
                                                                                gTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTg                                                                  <  1:1995499/150‑1 (MQ=255)
                                                                                 tCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTgg                                                                 >  2:8005694/1‑150 (MQ=255)
                                                                                  cGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGc                                                                <  2:5712629/150‑1 (MQ=255)
                                                                                  cGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGc                                                                <  1:4515590/150‑1 (MQ=255)
                                                                                     tGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGa                                                                         >  2:4963275/1‑138 (MQ=255)
                                                                                       gggAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGAc                                                                                 >  1:8174345/1‑128 (MQ=255)
                                                                                       gggAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATcg                                                           >  1:2940738/1‑150 (MQ=255)
                                                                                        ggAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATcgc                                                          <  2:2310953/150‑1 (MQ=255)
                                                                                         gAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATcgcg                                                         >  1:3371711/1‑150 (MQ=255)
                                                                                           aGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGaa                                                       <  1:6385943/150‑1 (MQ=255)
                                                                                              tCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGa                                                    >  2:3038839/1‑150 (MQ=255)
                                                                                              tCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGa                                                    >  2:3916835/1‑150 (MQ=255)
                                                                                              tCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGa                                                    >  2:4117389/1‑150 (MQ=255)
                                                                                              tCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGa                                                    >  2:4882521/1‑150 (MQ=255)
                                                                                               ccAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGAc                                                   >  2:3681602/1‑150 (MQ=255)
                                                                                                cAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACt                                                  >  1:1395620/1‑150 (MQ=255)
                                                                                                 aaGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTc                                                 >  2:4612701/1‑150 (MQ=255)
                                                                                                    tGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTc                                                                                                        >  1:5586208/1‑92 (MQ=255)
                                                                                                    tGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTc                                                                                                        <  2:5586208/92‑1 (MQ=255)
                                                                                                        aaaaCCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCTACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTAc                                          <  1:885312/150‑1 (MQ=255)
                                                                                                        aaaaCCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTAc                                          >  2:7633072/1‑150 (MQ=255)
                                                                                                         aaaCCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACg                                         <  1:2748576/150‑1 (MQ=255)
                                                                                                         aaaCCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACg                                         >  1:7141377/1‑150 (MQ=255)
                                                                                                             cGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCt                                     >  2:3601590/1‑150 (MQ=255)
                                                                                                             cGGCTGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCt                                     >  1:2195793/1‑150 (MQ=255)
                                                                                                                 tGGTACGTACGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTcgcg                                 >  1:1319052/1‑150 (MQ=255)
                                                                                                                   gtacgtacGACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGc                               >  2:9443418/1‑150 (MQ=255)
                                                                                                                    tacgtacgACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCt                              >  1:2093923/1‑150 (MQ=255)
                                                                                                                    tacgtacgACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCt                              <  1:6669333/150‑1 (MQ=255)
                                                                                                                       gtacgACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGc                           <  2:7547027/150‑1 (MQ=255)
                                                                                                                        tacgACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAAt                                                      <  2:4815913/122‑1 (MQ=255)
                                                                                                                         acgACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAAt                                                      >  1:2289335/1‑121 (MQ=255)
                                                                                                                           gACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTAt                       <  2:5871677/150‑1 (MQ=255)
                                                                                                                           gACCCACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTAt                       <  1:2622737/150‑1 (MQ=255)
                                                                                                                              ccACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGt                                                                                         >  1:4195041/1‑81 (MQ=255)
                                                                                                                              ccACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCt                              <  2:903935/140‑1 (MQ=255)
                                                                                                                              ccACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCt                              >  1:903916/1‑140 (MQ=255)
                                                                                                                               cACCGTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAACACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGac                   >  1:4401655/1‑150 (MQ=255)
                                                                                                                                   gTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGaccacc               >  1:7791774/1‑150 (MQ=255)
                                                                                                                                   gTCTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGaccacc               >  1:9325515/1‑150 (MQ=255)
                                                                                                                                     cTACACCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGACCACCGa             <  2:3325140/150‑1 (MQ=255)
                                                                                                                                         acCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGaccaccatggct         >  2:2689278/1‑144 (MQ=255)
                                                                                                                                         acCAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGACCACCGATCTg         >  2:2400190/1‑150 (MQ=255)
                                                                                                                                           cAACGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGACCACCGATCTGGc       >  2:8868383/1‑150 (MQ=255)
                                                                                                                                           cAACGTCAACAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCaa                                                                                     >  2:1760630/1‑72 (MQ=255)
                                                                                                                                           cAACGTCAACAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCaa                                                                                     <  1:1760630/72‑1 (MQ=255)
                                                                                                                                             aCGTCATCAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGACCACCGATCTGGCTc     >  2:1101736/1‑150 (MQ=255)
                                                                                                                                                tcatcaACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGACCACCGATCTGGCTcgcg  <  2:9281930/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCCCCTAACGACCAAGGCTCAGTTTGAGCAGGTTCATCGTTACAACGAGCTGGCCATCCAGGAGGGGGCTCATTGCTTGGTCGGTGGGGAAGCTCCAAGTGACAAAACCGGCTGGTACGTACGACCCACCGTCTACACCAACGTCAACAACTCGATGCGGATTGCTCGGGAAGAAATTTTCGGACCCGTTCTGGTACTGATTCCGTTCAAGGACGAAAACGAGGCGGTGGCCATCGCGAATGACTCGGACTACGGGCTCGCGGCTGGCGTATGGACCACCGATCTGGCTCGCG  >  NC_002947/2209328‑2209621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A