Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F85 I1 R1 23 334.9 15793868 95.8% 15130525 148.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,043,352 (G)10→11 coding (1120/1143 nt) rffE ← UDP‑N‑acetylglucosamine‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,043,3421.G78.5% 214.8 / 63.0 79coding (1130/1143 nt)rffEUDP‑N‑acetylglucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base . (17/0);  new base G (61/1);  total (78/1)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

GTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTT  >  NC_002947/2043195‑2043486
                                                                                                                                                      |                                                                                                                                                   
gtgCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCCCGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑Gg‑g‑‑gg                                                                                                                                                  >  1:7323540/1‑150 (MQ=255)
gtgCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGTGC‑Gg‑g‑‑gg                                                                                                                                                  >  1:7323337/1‑150 (MQ=255)
 tgCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑ggg                                                                                                                                                 >  1:7536697/1‑150 (MQ=255)
  gCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑gggg                                                                                                                                                >  1:5914145/1‑150 (MQ=255)
  gCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑gggg                                                                                                                                                >  1:3067140/1‑150 (MQ=255)
     ttCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑ggggggg                                                                                                                                             >  1:906022/1‑150 (MQ=255)
     ttCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑ggggggg                                                                                                                                             >  1:3689496/1‑150 (MQ=255)
                 tctcTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTgg                                                                                                                                 >  1:5576213/1‑150 (MQ=255)
                    ctTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTTGccc                                                                                                                              >  1:1721087/1‑149 (MQ=255)
                        gtcgtGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGc                                                                                                                         >  1:1574227/1‑150 (MQ=255)
                        gtcgtGATTGTTGACTGTTGTT‑GGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCtt                                                                                                                       >  1:1590422/1‑150 (MQ=255)
                        gtcgtGATTGTTGACTGTTGTT‑GGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCtt                                                                                                                       >  1:1590112/1‑150 (MQ=255)
                        gtcgtGATTGTCGACTGTTGTT‑GGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCtt                                                                                                                       >  1:1590319/1‑150 (MQ=255)
                                       tgttgtTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATgcgcgc                                                                                                          >  1:3812681/1‑150 (MQ=255)
                                       tgttgtTGGGGGGGCACGCAGAAGGGGGGCTGCTGGCCAG‑TTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATgcgcgcg                                                                                                         >  1:2881109/1‑150 (MQ=255)
                                            ttgGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGc                                                                                                    >  1:1913577/1‑150 (MQ=255)
                                                gggggCACGCGGAAGGGGGGCTGCTGGCCAGTTTTCACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TGGGGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCtt                                                                                                  >  1:2791922/1‑150 (MQ=255)
                                                  gggCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGcc                                                                                              >  1:5106126/1‑150 (MQ=255)
                                                      aCGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATc                                                                                           >  1:6040107/1‑150 (MQ=255)
                                                      aCGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATc                                                                                           >  1:6040076/1‑150 (MQ=255)
                                                                ggggCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAgggg                                                                                 >  1:3078127/1‑150 (MQ=255)
                                                                ggggCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAgggg                                                                                 >  1:4285333/1‑150 (MQ=255)
                                                                ggggCGGCGGGCCAGTTGTTAGTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGG‑G‑‑GGGGGGGGGGCCC‑GGTGGCGC‑GGGCTGTGCGGCTGCGCGCGCAGGCTTTGCCCTCGCCCTAgggg                                                                                 >  2:7359478/1‑150 (MQ=255)
                                                                 gggCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑CGTTGCCC‑GGGCATTTCCTAATCCCCCGCCGGCTTTTCCAAACCCAAAGGGGt                                                                                >  1:207582/1‑150 (MQ=255)
                                                                   gctgctGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTa                                                                              >  1:2271515/1‑150 (MQ=255)
                                                                       ctgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATggg                                                                          >  1:5929554/1‑150 (MQ=255)
                                                                       ctgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGCGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGGCACGGTGCCCA‑GGGGTTTGCGAATGCGCGCCACGCCTTGGCCATCCCCAGAGGGGTAATggg                                                                          >  2:3640834/1‑150 (MQ=255)
                                                                        tgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTTGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑gggggggg                                                                                                                                            <  2:5738162/84‑1 (MQ=255)
                                                                        tgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTTGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑gggggggg                                                                                                                                            >  1:5738162/1‑84 (MQ=255)
                                                                        tgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TGGGGGGGGGGGGGCA‑AGTTGCCACGGGATTTGCGAATCCGCCCCAAGGCTTTGCAAACCCCAAAGGGGTTAggg                                                                           >  1:3328214/1‑150 (MQ=255)
                                                                          gcgaGTTTTTACTGGGGGGCGGCGGGGCTGGGGTTATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGGCA‑GGTGGCGG‑GGGGTTTGCGTTTGGGGGCGCAGGCTTTGCGTTGGGCGTGGGGGTTATgggggt                                                                       >  2:5354208/4‑147 (MQ=255)
                                                                            cAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTAGTCGCGGGTGAACCCGCACCTCCATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGGAGGCGCGCGCCGGCTTTGCCAGCGCCAAAGGGGTTATGGGCGAAg                                                                     >  2:3801637/1‑150 (MQ=255)
                                                                              gTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCC‑GGTGGCCA‑GGGCTTTGCGTAGGCGCCCACCGCCTTTGCCAACGCCCACGGGGTGATGGGCGAAgat                                                                   >  2:6066024/1‑148 (MQ=255)
                                                                              gTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCt                                                                   >  1:2864378/1‑150 (MQ=255)
                                                                              gTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCt                                                                   >  1:3964230/1‑150 (MQ=255)
                                                                              gTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGAATTCGCGGGTGAACCAGAAACTACATAGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCAA‑GGGCTTTGCGAATGCGCGCACAGGCTTTGCCATCGCCAAAGGGGTAATGGGCGACGCt                                                                   >  2:7080109/1‑150 (MQ=255)
                                                                                 tttACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCAtt                                                               >  1:7247567/1‑150 (MQ=255)
                                                                                 tttACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGA‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCCCGCGCAGGCGTAGCCATCGCCATAGGGGTTATGGGCGACGCTCAt                                                                >  2:4078468/1‑150 (MQ=255)
                                                                                   tACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGTGC‑GG‑TGGGGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTTCGGATGCGCGCGCCGGCATTGCCAACCCCAGAGGGGGTATGGGCGAAGCTCAtt                                                               >  2:5400086/1‑150 (MQ=255)
                                                                                      tGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TGGGGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTcg                                                            >  1:5457116/1‑150 (MQ=255)
                                                                                      tGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTcgc                                                           >  1:521793/1‑150 (MQ=255)
                                                                                      tGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTcgc                                                           >  1:2445878/1‑150 (MQ=255)
                                                                                      tGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTcgc                                                           >  1:707650/1‑150 (MQ=255)
                                                                                              ggcggGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCCGAGGGGTTATGGGCGAAGCTCATTTCGCGATAcgcc                                                   >  2:1791421/1‑150 (MQ=255)
                                                                                                 ggggCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACgccgcc                                                >  2:2296232/1‑150 (MQ=255)
                                                                                                    gCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCa                                             >  1:593824/1‑150 (MQ=255)
                                                                                                      tGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATc                                           >  1:2466944/1‑150 (MQ=255)
                                                                                                      tGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATc                                           >  1:3315501/1‑150 (MQ=255)
                                                                                                       gCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGGTTGCGCGCGCAGGCTTTGCCATCGCCCTAGGGGTTATTGGCGAAGCGCATTTCGCGATAAGCCGCCACAtag                                          >  1:2420236/1‑148 (MQ=255)
                                                                                                        cGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TGGGGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCCCCGCATCg                                          >  1:4569629/1‑150 (MQ=255)
                                                                                                              tCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGG‑G‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCAGCGGTCAGCa                                   >  2:5624945/1‑150 (MQ=255)
                                                                                                              tCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TGGGGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGc                                    >  1:408611/1‑150 (MQ=255)
                                                                                                              tCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TGGGGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCGCATCGGTCAGc                                    >  1:408671/1‑150 (MQ=255)
                                                                                                              tCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCC‑GGTGGGCA‑GGGCGTTGCGTATGCGCGCGCCGGCTTTGCCATCGCCATTGGGGGTATGGGCGAAGCTCATTTCGCGATAAGCCGCCTCATCGGgcagca                                   >  2:358026/1‑150 (MQ=255)
                                                                                                              tCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCa                                   >  1:5894773/1‑150 (MQ=255)
                                                                                                               tgtaggtgcgGGTTCACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCaa                                  >  1:3430460/8‑150 (MQ=255)
                                                                                                                gTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAg                                 >  1:971391/1‑150 (MQ=255)
                                                                                                                gTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCGTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAACCTCATTTCGCGATACGCCGCCTCAGCGGTCAGCAAg                                 >  2:7561793/1‑150 (MQ=255)
                                                                                                                 tATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAgg                                >  2:7065933/1‑150 (MQ=255)
                                                                                                                 tATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCCCGCCGGCTTTGCCATCGCCATCGGGGGTAGGGGCGAGGCGCATTTCGCGGTACGCCGCCTCATCGGGCAGCCAgg                                >  2:5104084/1‑150 (MQ=255)
                                                                                                                  aggtgcgGGTTCACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCATTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCACATTTCGCGATACGCCGCCTCAGCGGGCAGCACGGt                               >  2:2421905/5‑150 (MQ=255)
                                                                                                                   ttCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTa                              >  1:4648783/1‑150 (MQ=255)
                                                                                                                    gtgcgGGTTCACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCC‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGGCAGCAAgggcc                             >  2:6578120/3‑147 (MQ=255)
                                                                                                                      gcgGGTGAACCCGCACCTACATTGAGC‑GG‑TGGGGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTAca                            >  1:715524/1‑149 (MQ=255)
                                                                                                                            gAACCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGcc                     >  1:7287895/1‑150 (MQ=255)
                                                                                                                            gAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGccc                    >  1:345764/1‑150 (MQ=255)
                                                                                                                            gAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGccc                    >  1:4286040/1‑150 (MQ=255)
                                                                                                                              aCCCGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCg                   >  1:3118167/1‑150 (MQ=255)
                                                                                                                               cccGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTAAACAACGCCCGc                  >  2:6650890/1‑150 (MQ=255)
                                                                                                                                ccGCACCTACATTGAGC‑GGAG‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCGTAGGGGTTATGGGCGAAGCGCATTTCGCGAAACGCCCCCTCATCGGTCAGCAAGGTCCACAACCCCCGca                 >  2:6689957/1‑150 (MQ=255)
                                                                                                                                ccGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTGTGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGca                 >  1:7738170/1‑150 (MQ=255)
                                                                                                                                ccGCACCTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGATTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGca                 >  1:6273238/1‑150 (MQ=255)
                                                                                                                                  gCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGGCA‑GGGCTTTTCGTATGCCCCCCCACGCGTTTCCATCGCCCAAGGGGTTATGGGCCAACCACAATTACCCATACCCCGCCTCAACGGGAAGCAAGGTACACAAAGCCCGcacca              >  2:4166033/1‑148 (MQ=255)
                                                                                                                                    aCCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATc            >  1:1277710/1‑150 (MQ=255)
                                                                                                                                     ccTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATc            <  2:3118167/150‑1 (MQ=255)
                                                                                                                                      cTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCC‑GGGCTTTGCGTATTCGCGCGCAGGCTTTGCCATCGCCAGAGGGGTGATGGGCGAAGCTCATTTCGCGGTACGCCGCCGCATCGGTCCGCACGGTACTCGACGCCCGCACAATCg           >  2:6601546/1‑150 (MQ=255)
                                                                                                                                      cTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCTTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCg           >  2:1417226/1‑150 (MQ=255)
                                                                                                                                      cTACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCAAAGGGGTTATGGGCGAAGATCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCg           >  2:7690621/1‑150 (MQ=255)
                                                                                                                                       tACATTGAGC‑GG‑TGGGGGGGGGGGGCCA‑GGGGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCAACGCCATAGGGGTTATTGGCGAAGCACATTTCGCGATACGCCGCCTCATCGGTAAGCAAGGTCCACAACGCCCGCCCAACCg           >  2:4538502/1‑150 (MQ=255)
                                                                                                                                       tACATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGa          >  1:6474176/1‑150 (MQ=255)
                                                                                                                                       tACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGAt         >  2:6501366/1‑150 (MQ=255)
                                                                                                                                        aCATTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCAGAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGAt         >  2:5801328/1‑150 (MQ=255)
                                                                                                                                          aTTGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGc       >  1:3849053/1‑150 (MQ=255)
                                                                                                                                           ttGAGC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCt      >  1:4935319/1‑150 (MQ=255)
                                                                                                                                           ttGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTa     >  1:6392570/1‑150 (MQ=255)
                                                                                                                                               ggggg‑T‑‑GGGGGGGGGGCCA‑GCTGGCCA‑GGCCTTTGAGGATGCGCCCGCACGCTTTGCCAAAGCCCGAGTGGTAATGCGCGAAGCTCATTTCGCGATTCCAGGCCTAAGCGGTCAGCAAGGTACTCAACCCCCGCAAAATCTCTGCTaatg  >  2:5890551/4‑147 (MQ=255)
                                                                                                                                               gC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACtt  >  2:2935021/1‑150 (MQ=255)
                                                                                                                                               gC‑GG‑TG‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGATACtg  >  2:802268/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGC‑GG‑T‑‑GGGGGGGGGGCCA‑GGTGGCCA‑GGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTT  >  NC_002947/2043195‑2043486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A