Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A30 F85 I2 R1	24	413.5	17725300	96.1%	17034013	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,043,352	(G)_10→11	coding (1120/1143 nt)	rffE ←	UDP‑N‑acetylglucosamine‑2‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,043,342	1	.	G	86.8%	469.2 / 68.4	152	coding (1130/1143 nt)	rffE	UDP‑N‑acetylglucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand): ref base . (19/1); new base G (132/0); total (151/1)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

GCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTC  >  NC_002947/2043197‑2043487

gCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑ggg                                                                                                                                                 >  1:868457/1‑149 (MQ=255)

gCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑ggg                                                                                                                                                 >  1:2758466/1‑149 (MQ=255)

   ttCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCCGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑ggg‑ggg                                                                                                                                             >  1:5925089/1‑149 (MQ=255)

      cAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑ggg‑gggggg                                                                                                                                          >  1:2590243/1‑149 (MQ=255)

        aGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑ggg‑gggggggg                                                                                                                                        >  1:3119390/1‑148 (MQ=255)

          gCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑ggg‑ggggggggcc                                                                                                                                      >  1:8174785/1‑146 (MQ=255)

              aTCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGt                                                                                                                                   >  1:5743277/1‑149 (MQ=255)

               tctcTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTg                                                                                                                                  >  1:6217396/1‑149 (MQ=255)

                   ttCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCa                                                                                                                              >  1:4297559/1‑149 (MQ=255)

                        cgtGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCt                                                                                                                         >  1:8649297/1‑149 (MQ=255)

                          tGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCttt                                                                                                                       >  1:7109891/1‑149 (MQ=255)

                            aTTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGc                                                                                                                     >  1:7890511/1‑149 (MQ=255)

                            aTTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCg                                                                                                                    >  1:1573800/1‑149 (MQ=255)

                              tgttgACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGAAATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGt                                                                                                                   >  1:5232591/1‑149 (MQ=255)

                                    cTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTCTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATgcgc                                                                                                             >  1:8496893/1‑149 (MQ=255)

                                          ttgGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAg                                                                                                       >  1:4434038/1‑149 (MQ=255)

                                           tgGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAgg                                                                                                      >  1:3927380/1‑149 (MQ=255)

                                              gggggCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCtt                                                                                                   >  1:3805602/1‑149 (MQ=255)

                                                  gCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGcc                                                                                               >  1:2632375/1‑149 (MQ=255)

                                                   cACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCa                                                                                              >  1:8350158/1‑149 (MQ=255)

                                                   cACGCGGAAGGGGGGCTGCTGGCCAGTTTTTAATGGTGGGCGGCGGGGCTGCGGAAATCGTATTCGCGGGTGAACCCGCACCAACATTGA‑GC‑GGT‑‑‑GGGCGGGGGGGCCATGGGGCAAGCGCTTTGCCTATGAGCGCGCAGGCATTGCCa                                                                                              >  1:5377736/1‑149 (MQ=255)

                                                       cgGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGc                                                                                          >  1:8120197/1‑149 (MQ=255)

                                                       cgGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGc                                                                                          >  1:5790095/1‑149 (MQ=255)

                                                       cgGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGc                                                                                          >  1:5790063/1‑149 (MQ=255)

                                                       cgGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGcc                                                                                         >  1:2907899/1‑149 (MQ=255)

                                                         gAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTAAGCGCCCGCAGGCATTGCCAACGCCa                                                                                        >  1:1492507/1‑149 (MQ=255)

                                                            ggggggCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAg                                                                                     >  1:3329425/1‑149 (MQ=255)

                                                             gggggCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGAAGGCTTTGCCATCGCCATAgg                                                                                    >  1:7393139/1‑149 (MQ=255)

                                                                 gctgctGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCCGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGtt                                                                                >  1:790532/1‑149 (MQ=255)

                                                                    gctgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATg                                                                             >  1:7016832/1‑149 (MQ=255)

                                                                     ctgGCCAGTTTTTACTGGGTGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCAGAGGGGTTATgg                                                                            >  2:6120482/1‑149 (MQ=255)

                                                                      tgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGc                                                                          >  1:6131781/1‑149 (MQ=255)

                                                                       ggCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GCGGGG‑‑‑GGG‑GGGGGGGCCAGGGGGCCCGGGCTTTGGGTATGCGCGCCCCGGCTTTGCCAACGCCAGGGGGGTGATGGGc                                                                          >  2:5607156/1‑149 (MQ=255)

                                                                         ccAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGa                                                                        >  1:2888429/1‑149 (MQ=255)

                                                                         ccAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGa                                                                        >  1:7539375/1‑149 (MQ=255)

                                                                         ccAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGa                                                                        >  1:2888021/1‑149 (MQ=255)

                                                                          cAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCCGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCAAAGGGGTTATGGGCGaa                                                                       >  1:3924987/1‑149 (MQ=255)

                                                                           aGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCGTTGCCATCGCCATAGGGGTTATGGGCGAAg                                                                      >  1:7947145/1‑149 (MQ=255)

                                                                           aGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTCCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAg                                                                      >  1:3548524/1‑149 (MQ=255)

                                                                           aGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGc                                                                     >  2:4447575/1‑149 (MQ=255)

                                                                           aGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATGGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAg                                                                      >  2:4354025/1‑149 (MQ=255)

                                                                             tttttACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GCGGGG‑‑‑GGG‑GGGGGGGCCCGGTGGCCCGGGCTTTGCGTATGCGCGCGCAGGCGTTGCCATCGCCATAGGGGTTCAGGGCGCAGcg                                                                    >  2:5720351/1‑148 (MQ=255)

                                                                              ttttACTGGGGGGCGGCGGGGCTGCGGATATCGTCTTCGCGGGGGAACCCGCACCGACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCCGGGGGCCAGGGCGTTGCGTGGGCCCGCGGAGGCTTTGCCGTCGCCCTGGGGGGGCTGGGCGAAGCTc                                                                   >  2:2697420/1‑149 (MQ=255)

                                                                                ttACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGCACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCGGGGGGCCCGGGCTTTCCGTAGGCCCGCGCAGCCATTGCCAACGCCAGGGGGGTTAGGGGCGagggtatt                                                                 >  2:1669459/1‑142 (MQ=255)

                                                                                 tACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCAtt                                                                >  1:2348431/1‑149 (MQ=255)

                                                                                 tACTGGGGGGCCGCGGGGCTGCGGAAAAAGTAATCCCCGGTGAACCCCCACCAACAATGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTTCGTATTCGCGCGCACGCATTTCCAACGCCAAAGGGGTTAATGGCGAAGCTCaat                                                                >  1:3250462/1‑147 (MQ=255)

                                                                                   cTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTc                                                              >  1:252278/1‑149 (MQ=255)

                                                                                    tGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTcg                                                             >  1:4868878/1‑149 (MQ=255)

                                                                                     ggggggCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACCTTGAGGG‑GGG‑‑‑GGG‑GGGGGGGCCAGGTGGCCCGGGCGTTGCGGGGGCGCGCGCGGGCTTTGCCATCGGCCGGGGGGTGATGGGCGTCGCTCCTGTcgc                                                            >  2:5360101/1‑149 (MQ=255)

                                                                                     ggggggCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTcgc                                                            >  1:7007146/1‑149 (MQ=255)

                                                                                     ggggggCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTcgcg                                                           >  1:4051520/1‑149 (MQ=255)

                                                                                       ggggCGGCGGGGGTGCGGTTATCGTATTCGCGGGTGAACCCGCACCTTCATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCGGGTGGCCAGGGCGTTGGGTGTGCGGGGGCAGGCGTTGCCAGCGCGCTAGGGGTTTTGGGCGGAGCTCATTTcgcgg                                                          >  2:2216481/1‑148 (MQ=255)

                                                                                        gggCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGAt                                                         >  1:2628408/1‑149 (MQ=255)

                                                                                             gcggGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATAcgcc                                                    >  1:450673/1‑149 (MQ=255)

                                                                                              cggGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATAcgccg                                                   >  1:2440659/1‑149 (MQ=255)

                                                                                                gggCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGGTTTGCGTATGCGCGCGCCGGCTTTGCCATCGCCATGGGGGTTATGGGCGACGCTCATGTCGCGATAGgccgcc                                                 >  2:3022154/1‑149 (MQ=255)

                                                                                                     gCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTGG‑GGG‑GGGGGGGCCAGGTGGCAAGGGCTTAGCGTATGCGCGCGAAGGCTTTGACATCGCCATAGGGGTTATGGGCTAAGCTCATTTAGCGATAACCCGCCTCAt                                             >  2:5719323/1‑149 (MQ=255)

                                                                                                      cGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTTTGGGCGAAGCGCATTTAGCGATACGCCGCCGCATCg                                           >  2:2866458/1‑149 (MQ=255)

                                                                                                      cGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCg                                           >  1:5913240/1‑149 (MQ=255)

                                                                                                      cGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCg                                           >  1:6534764/1‑149 (MQ=255)

                                                                                                        gATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGAATGCGCGCGCAGGATTTGCCATCGCCCTAGGGGTTATGGGCGAAGCTCATTTAGCGATACGCCGCCTCATCGGt                                         >  2:3593441/1‑149 (MQ=255)

                                                                                                           atCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAg                                      >  1:1339823/1‑149 (MQ=255)

                                                                                                            tCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCACGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCAATCGCCAAAGGGGTTATTGGCCAACCTCATGTCCCGATACGCCGCCTCATCGGTCAGc                                     >  1:2706996/1‑149 (MQ=255)

                                                                                                             cGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCa                                    >  1:2050717/1‑149 (MQ=255)

                                                                                                             cGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCaa                                   >  1:1110439/1‑149 (MQ=255)

                                                                                                              gTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGAAGGCTTTGCCATCGCCAGAGGGGGTATGGGCGACGCTCATTTCGCGTTACGCCGCATCAGCGGGCAGCaa                                   >  2:3642847/1‑149 (MQ=255)

                                                                                                               taggtgcgGGTTCACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCGTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATCGGGGTTAGGGGCGAAGCTCATTTCGCGATACGCCGCCTCAGCGGTCAGCGAg                                  >  2:8070375/6‑149 (MQ=255)

                                                                                                               tATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAg                                  >  1:8620236/1‑149 (MQ=255)

                                                                                                               tATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCAAAGGGGTTATGGGCCAAGCTCATGTCGCGATACGCCGCCTCATCGGTCAGCacg                                  >  2:4554369/1‑147 (MQ=255)

                                                                                                                 ttCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCGTAGCGTATGCGCCCGCAGGAGTTGCCAACGCCAAACGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGt                                >  2:345470/1‑149 (MQ=255)

                                                                                                                   tgcgGGTTCACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTAc                              >  1:1082359/2‑149 (MQ=255)

                                                                                                                    gcgGGTTCACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACt                             >  1:6796146/1‑149 (MQ=255)

                                                                                                                    gcgGGTTCACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCGTTGCGGATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCGCAGCGGTCAGCAAGGTAcg                             >  2:4314446/1‑148 (MQ=255)

                                                                                                                     cgGGTGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTAGCGAAACGCCGCCTCATAGGTCAGACATGTACTc                            >  2:7865943/1‑149 (MQ=255)

                                                                                                                         tggaCCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACg                        >  2:7320511/4‑149 (MQ=255)

                                                                                                                         tGAACCCGCACCTACATTGA‑GC‑GGTGGGGGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTaaa                          >  1:6458706/1‑149 (MQ=255)

                                                                                                                         tGAACCCGCACCTACATTGA‑GC‑GGTGG‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAAc                         >  1:7782068/1‑149 (MQ=255)

                                                                                                                         tGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACg                        >  1:6917516/1‑149 (MQ=255)

                                                                                                                         tGAACCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACg                        >  2:6197634/1‑149 (MQ=255)

                                                                                                                           aaCCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTCCTCAACGcc                      >  2:5154426/1‑149 (MQ=255)

                                                                                                                           aaCCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGcc                      >  2:4242346/1‑149 (MQ=255)

                                                                                                                            aCCCGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGccc                     >  1:6634137/1‑149 (MQ=255)

                                                                                                                             cccGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCg                    >  2:6598666/1‑149 (MQ=255)

                                                                                                                             cccGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCg                    >  2:4077290/1‑149 (MQ=255)

                                                                                                                             cccGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATAGCCATAGGGGTTATTGGCGAAGCACATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCg                    >  1:1327131/1‑149 (MQ=255)

                                                                                                                              ccGCACCTACATTGA‑GC‑GGTGG‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATAGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATAGGTCAGCAAGGTAATCAACGCCCg                    >  2:3097840/1‑149 (MQ=255)

                                                                                                                               cGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGca                  >  1:5651314/1‑149 (MQ=255)

                                                                                                                               cGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGca                  >  1:5650870/1‑149 (MQ=255)

                                                                                                                               cGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGGTATGGGCGAAGCTCATTGCGCGATACGCCGCCGCAGCGGTCAGCAAGGTACTCAccgcccgcc                  >  2:6024069/1‑148 (MQ=255)

                                                                                                                               cGCACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCACGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGca                  >  2:1545932/1‑149 (MQ=255)

                                                                                                                                 cACCTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTAAGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCCCCTAAAAGGTCAGCAAGGAACAAAACGCCCCcaca                >  2:2879968/1‑149 (MQ=255)

                                                                                                                                 cACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACaa               >  1:480262/1‑149 (MQ=255)

                                                                                                                                   ccTACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAAt              >  1:1674220/1‑149 (MQ=255)

                                                                                                                                     tACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCg            >  1:277881/1‑149 (MQ=255)

                                                                                                                                     tACATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCACATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCg            >  1:5883538/1‑149 (MQ=255)

                                                                                                                                      aCATTGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGa           >  2:6369835/1‑149 (MQ=255)

                                                                                                                                          tGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCCGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTGTGCCATCGCCATGGGGGTTATGGGCGGAGCTCATTTCGCGATACGCCGCCGCATCGGTCAGCAAGGTACTCAGCGCCCGAACAAACGATGCt       >  2:2501382/1‑149 (MQ=255)

                                                                                                                                          tGA‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGAATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCGCAGCGGTCAGCCCGGTACTCAACGCCCGCACAATCGATGCt       >  2:122575/1‑149 (MQ=255)

                                                                                                                                            a‑GC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCACAACGGTCAGCAAGGTCCTCAACGCCCGCACAATCGATGCTAc     >  2:6922953/1‑149 (MQ=255)

                                                                                                                                              gC‑GGTG‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACt    >  1:2293228/1‑149 (MQ=255)

GCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGA‑GC‑GGT‑‑‑GGG‑GGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTC  >  NC_002947/2043197‑2043487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A