Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,927,903 |
G→A |
intergenic (+231/‑326) |
ppnN → / → sdaC |
nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,927,903 | 0 | G | A | 100.0%
| 99.5
/ NA
| 28 | intergenic (+231/‑326) | ppnN/sdaC | nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/12); total (16/12) |
CAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCT > NC_000913/2927825‑2927979
|
cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAAt < 1:529586/81‑1 (MQ=255)
tttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTc < 1:529587/81‑1 (MQ=255)
tttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTc < 2:529588/81‑1 (MQ=255)
ttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAAtttttt > 1:529614/1‑79 (MQ=255)
ttCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAAcc > 1:529616/1‑80 (MQ=255)
aTAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACa > 1:529624/1‑81 (MQ=255)
tAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACat < 1:705128/81‑1 (MQ=255)
gTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTg > 1:529613/1‑81 (MQ=255)
tATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGcc > 2:529621/1‑81 (MQ=255)
tCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGccc < 2:529589/80‑1 (MQ=255)
gCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCg < 1:529590/80‑1 (MQ=255)
aTGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCgg > 2:529625/1‑78 (MQ=255)
tGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaa < 1:529591/79‑1 (MQ=255)
aaGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGt > 2:529631/1‑79 (MQ=255)
aGAAATTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAg < 1:529592/81‑1 (MQ=255)
aaaTTATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGAt > 2:529619/1‑81 (MQ=255)
tATCGCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCg > 2:529629/1‑81 (MQ=255)
gCCATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTa < 2:529593/81‑1 (MQ=255)
cATGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAAt > 1:529612/1‑81 (MQ=255)
aTGATTAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATg > 1:529620/1‑81 (MQ=255)
tAACTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCa > 2:529618/1‑80 (MQ=255)
aCTAAAAGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCaaa > 2:529628/1‑80 (MQ=255)
aaaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATAtt > 2:529615/1‑81 (MQ=255)
aaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAatat < 1:529595/79‑1 (MQ=255)
aaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATAtt < 2:529594/80‑1 (MQ=255)
aaaGTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTg < 2:529596/81‑1 (MQ=255)
gTATTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGccc > 2:529636/1‑81 (MQ=255)
aTTAATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCt > 1:529623/1‑80 (MQ=255)
|
CAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCT > NC_000913/2927825‑2927979
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
