Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,940,603 |
Δ1 bp |
coding (641/1101 nt) |
rlmM ← |
23S rRNA 2'‑O‑ribose C2498 methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,940,601 | 0 | C | . | 100.0%
| 142.9
/ NA
| 33 | coding (643/1101 nt) | rlmM | 23S rRNA 2'‑O‑ribose C2498 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (14/19); total (14/19) |
GTTGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGT > NC_000913/2940521‑2940671
|
gTTGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGc < 2:532419/81‑1 (MQ=255)
ttGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcc < 2:532420/81‑1 (MQ=255)
tGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcca > 1:532486/1‑80 (MQ=255)
tGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcca > 2:532461/1‑80 (MQ=255)
tGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcca > 2:532421/1‑80 (MQ=255)
tCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGccaca < 1:532422/81‑4 (MQ=255)
gACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATc > 2:532476/1‑81 (MQ=255)
aCGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATcc > 1:532472/1‑81 (MQ=255)
cGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATccc < 1:532423/81‑1 (MQ=255)
aggAATAAAAAAAAATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATccc < 1:941309/80‑1 (MQ=255)
ggAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCg > 2:532477/1‑81 (MQ=255)
aCCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGc > 1:532473/1‑79 (MQ=255)
cccACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAg < 1:532424/81‑1 (MQ=255)
cTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCAc < 1:532425/81‑1 (MQ=255)
tCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACt > 1:532479/1‑80 (MQ=255)
cACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACt < 1:532426/79‑1 (MQ=255)
cAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATcc < 2:532427/81‑1 (MQ=255)
aGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg > 1:532526/1‑81 (MQ=255)
gTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg > 1:532484/1‑80 (MQ=255)
gTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg > 1:532469/1‑80 (MQ=255)
ggTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAg > 2:532488/1‑80 (MQ=255)
gTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAgg < 1:532429/80‑1 (MQ=255)
gTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CAACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAgg < 1:532428/80‑1 (MQ=255)
ggTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa < 1:532431/81‑1 (MQ=255)
ggTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa < 2:532432/81‑1 (MQ=255)
ggTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa < 2:532430/81‑1 (MQ=255)
gTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATaa < 2:532433/81‑1 (MQ=255)
gTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATaa < 1:532434/81‑1 (MQ=255)
gTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa > 2:532492/1‑80 (MQ=255)
ccAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGa > 2:532483/1‑80 (MQ=255)
aGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAAt > 1:532497/1‑81 (MQ=255)
cAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCct > 2:532480/1‑81 (MQ=255)
aaGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCctc < 1:532435/81‑1 (MQ=255)
aGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCctct < 1:532436/81‑1 (MQ=255)
aGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCctct > 1:532485/1‑81 (MQ=255)
gcgcCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCtt < 1:532437/81‑1 (MQ=255)
gcgcCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCtt < 1:532438/81‑1 (MQ=255)
gcCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTcc < 1:532439/81‑1 (MQ=255)
aaTCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGt < 1:532440/79‑1 (MQ=255)
|
GTTGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGT > NC_000913/2940521‑2940671
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
