Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,945,771 |
C→T |
A24A (GCG→GCA) |
tcdA ← |
tRNA threonylcarbamoyladenosine dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,945,771 | 0 | C | T | 100.0%
| 76.0
/ NA
| 22 | A24A (GCG→GCA) | tcdA | tRNA threonylcarbamoyladenosine dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/15); total (7/15) |
CCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA > NC_000913/2945695‑2945846
|
ccAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCtt < 2:480611/81‑1 (MQ=255)
cgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAg < 1:480613/81‑1 (MQ=255)
cgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAg < 2:480612/81‑1 (MQ=255)
cAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgc > 2:480658/1‑81 (MQ=255)
cAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgc > 2:480648/1‑81 (MQ=255)
aGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgcg > 2:480661/1‑81 (MQ=255)
gACACCACCGATACCGACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGc > 2:480655/1‑80 (MQ=255)
ccGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCaaaa < 1:480614/81‑1 (MQ=255)
aTACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGc < 2:480615/81‑1 (MQ=255)
aaaTATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCAt < 1:480616/81‑1 (MQ=255)
tGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTa < 2:480617/81‑1 (MQ=255)
gcgTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAAtt < 2:480618/81‑1 (MQ=255)
cgTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTa < 1:480619/81‑1 (MQ=255)
gTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAc < 1:480620/81‑1 (MQ=255)
tCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAcc < 2:480622/81‑1 (MQ=255)
tCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAcc < 1:480621/81‑1 (MQ=255)
cAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAg < 1:480623/81‑1 (MQ=255)
aaaCAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGa > 2:480665/1‑81 (MQ=255)
aCAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACa > 1:480663/1‑81 (MQ=255)
cAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACAt < 1:480625/81‑1 (MQ=255)
cAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACAt < 2:480624/81‑1 (MQ=255)
tGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCa > 1:480668/1‑81 (MQ=255)
|
CCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA > NC_000913/2945695‑2945846
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
