Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,945,771 |
C→T |
A24A (GCG→GCA) |
tcdA ← |
tRNA threonylcarbamoyladenosine dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,945,771 | 0 | C | T | 100.0%
| 82.8
/ NA
| 24 | A24A (GCG→GCA) | tcdA | tRNA threonylcarbamoyladenosine dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/13); total (11/13) |
CGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA > NC_000913/2945693‑2945846
|
cgcCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGc < 1:536252/81‑1 (MQ=255)
cTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCAc > 2:536297/1‑81 (MQ=255)
ccgccgCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCa > 2:536302/1‑80 (MQ=255)
ccgccgCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCa < 2:536253/80‑1 (MQ=255)
gccgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATa > 2:536286/1‑80 (MQ=255)
gccgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATAc < 2:536254/81‑1 (MQ=255)
ccgccCAGGAACCGACACCACCGATACCAACCACCCAAATATGCGCGTCAGCAAACAGATGCAATGCTTTTTCACCATa > 1:536285/1‑79 (MQ=255)
cccAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAc < 1:536255/81‑1 (MQ=255)
aGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgcg < 2:536256/81‑1 (MQ=255)
aaCCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCtg > 2:536257/1‑81 (MQ=255)
cGACGCCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGc < 1:536258/81‑1 (MQ=255)
caccGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCaa < 1:536259/81‑1 (MQ=255)
cGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAAc < 1:536260/81‑1 (MQ=255)
gATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACg > 1:536295/1‑81 (MQ=255)
tACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCt < 1:536261/81‑1 (MQ=255)
accaaccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCt > 1:536291/1‑80 (MQ=255)
caaccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGAc < 2:536262/81‑1 (MQ=255)
caaccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGAc > 2:536292/1‑81 (MQ=255)
ccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGcc < 2:536263/81‑1 (MQ=255)
aaTATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCAt > 2:536296/1‑80 (MQ=255)
tGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACt < 1:536264/80‑1 (MQ=255)
tCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAcc < 1:536265/81‑1 (MQ=255)
aGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACa > 2:536312/1‑79 (MQ=255)
tGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCa > 1:536314/1‑81 (MQ=255)
|
CGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA > NC_000913/2945693‑2945846
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
