Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,012,842 |
C→A |
E466D (GAG→GAT) |
yqeB ← |
XdhC‑CoxI family protein YqeB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,012,842 | 0 | C | A | 100.0%
| 162.2
/ NA
| 45 | E466D (GAG→GAT) | yqeB | XdhC‑CoxI family protein YqeB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/23); total (22/23) |
TAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGC > NC_000913/3012766‑3012919
|
tAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCAc > 1:547448/1‑81 (MQ=255)
aaaaCCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCAc > 2:547460/1‑80 (MQ=255)
aaaCCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCAcc > 1:547449/1‑80 (MQ=255)
aaCCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCaa > 1:547454/1‑81 (MQ=255)
ccaaccaCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAg < 1:547394/81‑1 (MQ=255)
caaccaCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAgg > 2:547453/1‑81 (MQ=255)
aaccaCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAgg > 1:547457/1‑80 (MQ=255)
caCTGCCAGGCCGTCGTTCAACAAGCCACGCGCCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAAt < 2:547395/81‑1 (MQ=255)
aCTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATc < 2:547396/81‑1 (MQ=255)
gCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATcaca > 2:547458/1‑81 (MQ=255)
cAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATc < 1:547397/81‑1 (MQ=255)
cAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATc < 2:547398/81‑1 (MQ=255)
aGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCg > 1:547451/1‑81 (MQ=255)
gCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGcc < 1:547399/81‑1 (MQ=255)
gCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGcc > 2:547455/1‑81 (MQ=255)
ccGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGccc < 2:547400/81‑1 (MQ=255)
tcgtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCctct > 2:547450/1‑81 (MQ=255)
gtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCttt < 1:547402/81‑1 (MQ=255)
gtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCttt > 1:547470/1‑81 (MQ=255)
gtTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCtt < 2:547401/80‑1 (MQ=255)
ttCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCttt > 1:547478/1‑80 (MQ=255)
aacaaGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCAcc < 1:547403/81‑1 (MQ=255)
caaGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAg < 1:547404/81‑1 (MQ=255)
gCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATc < 2:547405/81‑1 (MQ=255)
cACCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTaa > 1:547452/1‑81 (MQ=255)
aCCATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAt > 1:547474/1‑81 (MQ=255)
ccATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAtt < 1:547408/81‑1 (MQ=255)
ccATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAtt < 2:547407/81‑1 (MQ=255)
ccATCCCCGTCAACGGCGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAtt < 2:547406/81‑1 (MQ=255)
cATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt > 2:547463/1‑81 (MQ=255)
cATCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt < 1:547409/81‑1 (MQ=255)
aTCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAAttt > 2:547459/1‑80 (MQ=255)
tCCCCGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCa > 2:547447/1‑81 (MQ=255)
cccGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCat < 1:547411/80‑2 (MQ=255)
cccGTCAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCAc < 1:547410/80‑1 (MQ=255)
cAACGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGa > 1:547491/1‑81 (MQ=255)
aaCGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGAt > 2:547468/1‑81 (MQ=255)
aaCGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGAt < 1:547412/81‑1 (MQ=255)
aCGGTGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATc < 2:547413/81‑1 (MQ=255)
tGCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCa > 2:547464/1‑80 (MQ=255)
gCTTTAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCAt > 1:547465/1‑80 (MQ=255)
tAATTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAAt < 1:547414/79‑1 (MQ=255)
aaTTTCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGcc > 2:547481/1‑81 (MQ=255)
tttCATGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTg < 2:547415/81‑1 (MQ=255)
aTGATCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTgcagc < 1:547416/81‑1 (MQ=255)
|
TAAAACCACCAACCACTGCCAGGCCGTCGTTCAACAAGCCACGCACCATCCCCGTCAACGGTGCTTTAATTTCATGCTCACCAATCCAGGCAATCACATCGCCCTCTTTCACCAGATCGCCTAATTTCACGTTGGATCGCATAATGCCTGCAGC > NC_000913/3012766‑3012919
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
