Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,099,719 |
T→A |
D337V (GAT→GTT) |
ansB ← |
L‑asparaginase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,099,719 | 0 | T | A | 100.0%
| 140.3
/ NA
| 40 | D337V (GAT→GTT) | ansB | L‑asparaginase 2 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (24/16); total (24/16) |
CTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCC > NC_000913/3099641‑3099795
|
cTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACt > 2:567818/1‑81 (MQ=255)
aaGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACttt < 2:567764/80‑1 (MQ=255)
gTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGt < 1:567765/81‑1 (MQ=255)
gTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGt > 1:567791/1‑81 (MQ=255)
aaGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGt > 1:567809/1‑81 (MQ=255)
gCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCa < 1:567766/81‑1 (MQ=255)
ccGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCagag < 2:567768/81‑1 (MQ=255)
ccGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCagag < 1:567767/81‑1 (MQ=255)
cGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGc < 1:567769/81‑1 (MQ=255)
ggCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGc > 1:567812/1‑80 (MQ=255)
cGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGtt < 1:567770/81‑1 (MQ=255)
aTACCGGGGCGCGGCGATTAGTACTGATTGAAGATCCGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGttt > 1:567813/1‑79 (MQ=255)
aTACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTgc < 2:567771/81‑1 (MQ=255)
ggggCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTgcag > 1:567807/1‑78 (MQ=255)
cgaagcgaTTAGTACTGATTGTAGATCTGCTGGATCTGCTGCGGAACTTTGATTTGCGTCAGAACCAGTTGCAGCAGAAc < 2:567772/80‑1 (MQ=255)
gaggcgaTTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAAcgc > 1:567819/1‑81 (MQ=255)
gaTTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCt > 2:567808/1‑81 (MQ=255)
ttAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttt > 1:567810/1‑81 (MQ=255)
ttAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttt > 1:567820/1‑81 (MQ=255)
ttAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttt < 1:567773/81‑1 (MQ=255)
aGTACTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCttttt > 1:567800/1‑81 (MQ=255)
aCTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCg > 2:567805/1‑81 (MQ=255)
aCTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCg > 1:567823/1‑81 (MQ=255)
aCTGATTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCg > 1:567816/1‑81 (MQ=255)
aTTGAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGtt > 2:567815/1‑81 (MQ=255)
gAAGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTCCGGGTTCAg > 2:567814/1‑81 (MQ=255)
aGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCg > 2:567826/1‑81 (MQ=255)
aGATCTGCTGGATCTGCTGCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCg > 2:567811/1‑81 (MQ=255)
gatctgctggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGt < 1:567774/81‑1 (MQ=255)
gctggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGc > 2:567829/1‑78 (MQ=255)
ctggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCag < 1:567775/80‑1 (MQ=255)
ggatctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAgg > 2:567806/1‑81 (MQ=255)
ctgctgCGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgac > 1:567840/1‑81 (MQ=255)
gctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgacg > 2:567802/1‑80 (MQ=255)
gctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgacg > 2:567817/1‑80 (MQ=255)
ctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgacga < 2:567777/80‑1 (MQ=255)
ctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcgac < 1:567778/78‑1 (MQ=255)
ctgcGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGcg < 1:567776/76‑1 (MQ=255)
cGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGcc < 2:567779/81‑1 (MQ=255)
cGGAACTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGcc > 2:567838/1‑81 (MQ=255)
|
CTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCC > NC_000913/3099641‑3099795
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
