Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,119,306 |
T→A |
intergenic (‑194/+291) |
yghJ ← / ← glcA |
putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,119,306 | 0 | T | A | 100.0%
| 55.3
/ NA
| 16 | intergenic (‑194/+291) | yghJ/glcA | putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (11/5); total (11/5) |
GAAATTTATAAGAATAACCCCTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTAACGATTAAGATTGCAAAAGCAACAGATAG > NC_000913/3119236‑3119381
|
gAAATTTATAAGAATAACCCCTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACAtt > 1:572204/1‑81 (MQ=255)
taAGAATAACCCCTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAAc < 2:572197/80‑1 (MQ=255)
gAATAACCCCTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTaa > 1:572220/1‑81 (MQ=255)
aaTAACCCCTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAAc > 1:572213/1‑81 (MQ=255)
aaCCCCTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACta > 1:572223/1‑80 (MQ=255)
ccccTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACtata > 1:572214/1‑80 (MQ=255)
ccccTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACtata > 1:572216/1‑80 (MQ=255)
gATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACTATATAAATttgtt < 1:572198/81‑1 (MQ=255)
gATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACTATATAAATttgtt > 1:572211/1‑81 (MQ=255)
gATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACTATATAAATttgtt > 1:572212/1‑81 (MQ=255)
tCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACTATATAAATTTGTTgg > 1:572219/1‑79 (MQ=255)
gCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGtttt < 2:572200/78‑1 (MQ=255)
gCTATTTTTACGAAATTTCAACAAACAAAAAAATAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTa < 1:572199/79‑1 (MQ=255)
aacaaaAAAATAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTAACGATTAAGATTGCAAAAGCaa > 2:572208/1‑78 (MQ=255)
aaaaaaATAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTAACGATTAAGATTGCAAAAGCAACAGATa < 2:572201/81‑1 (MQ=255)
aaaaATAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTAACGATTAAGATTGCAAAAGCAACAGATAg > 2:572222/1‑80 (MQ=255)
|
GAAATTTATAAGAATAACCCCTATACGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTAACGATTAAGATTGCAAAAGCAACAGATAG > NC_000913/3119236‑3119381
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
