Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,119,442 |
(A)7→6 |
intergenic (‑330/+155) |
yghJ ← / ← glcA |
putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,119,436 | 0 | A | . | 100.0%
| 99.1
/ NA
| 24 | intergenic (‑324/+161) | yghJ/glcA | putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (10/14); total (10/14) |
TAAGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATA > NC_000913/3119358‑3119510
|
tAAGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaa > 2:516483/1‑80 (MQ=255)
aaGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACACAATTAATACAGACTTGTTTTGCGTGATaaa > 2:516480/1‑80 (MQ=255)
aGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaa < 1:516461/81‑1 (MQ=255)
aTTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaaat < 2:516461/81‑2 (MQ=255)
ttGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaaa > 2:516486/1‑79 (MQ=255)
gCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaaatag < 1:516463/80‑4 (MQ=255)
aaaaGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATg > 2:516489/1‑81 (MQ=255)
gCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTtct > 1:516495/1‑81 (MQ=255)
gCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTtct < 2:516463/81‑1 (MQ=255)
aCAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCAc > 1:516482/1‑81 (MQ=255)
aCAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCAc < 2:516464/81‑1 (MQ=255)
aTAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGctc < 1:516466/81‑1 (MQ=255)
aGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGctct > 2:516491/1‑80 (MQ=255)
gAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCtt < 1:516467/80‑1 (MQ=255)
gAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTa < 1:516468/81‑1 (MQ=255)
cTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAg > 1:516494/1‑81 (MQ=255)
atcaAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGt < 2:516468/80‑1 (MQ=255)
aaGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTAt < 1:516470/80‑1 (MQ=255)
gCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCt > 2:516495/1‑80 (MQ=255)
gCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTg < 1:516471/81‑1 (MQ=255)
cGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTg > 2:516499/1‑80 (MQ=255)
cATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACg > 1:516491/1‑80 (MQ=255)
cATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGc < 2:516471/81‑1 (MQ=255)
aTAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCa < 1:516473/81‑1 (MQ=255)
gTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATtgctgc < 1:516474/79‑1 (MQ=255)
gTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCAt > 2:516503/1‑81 (MQ=255)
ttttGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCAt < 1:516476/80‑1 (MQ=255)
ttttGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATg < 2:516474/81‑1 (MQ=255)
gCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGat > 2:516492/1‑80 (MQ=255)
cGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGata < 1:516477/80‑1 (MQ=255)
|
TAAGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATA > NC_000913/3119358‑3119510
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
