Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,123,304 |
C→T |
A168T (GCC→ACC) |
glcB ← |
malate synthase G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,123,304 | 0 | C | T | 100.0%
| 153.7
/ NA
| 43 | A168T (GCC→ACC) | glcB | malate synthase G |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/16); total (27/16) |
CCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATA > NC_000913/3123226‑3123381
|
ccaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGa > 2:517243/1‑81 (MQ=255)
caccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGAt < 2:517223/81‑1 (MQ=255)
accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGAt > 2:517251/1‑80 (MQ=255)
ccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATaa < 1:517225/81‑1 (MQ=255)
cTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAAc > 1:517256/1‑81 (MQ=255)
aaaCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTg > 2:517270/1‑81 (MQ=255)
cGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCt > 2:517266/1‑80 (MQ=255)
gCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCa > 1:517254/1‑81 (MQ=255)
ccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACcgc > 1:517277/1‑81 (MQ=255)
ccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACcgc > 1:517292/1‑81 (MQ=255)
caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGtt > 2:517268/1‑81 (MQ=255)
tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGc < 2:517225/81‑1 (MQ=255)
cTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCgg < 2:517226/81‑1 (MQ=255)
cTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCg > 1:517279/1‑80 (MQ=255)
aTAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATc < 2:517227/81‑1 (MQ=255)
tAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCg > 1:517295/1‑81 (MQ=255)
ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGcc < 1:517229/80‑1 (MQ=255)
ttttCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTg < 2:517229/81‑1 (MQ=255)
ccAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGAcc > 2:517265/1‑80 (MQ=255)
cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt > 1:517293/1‑81 (MQ=255)
cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt < 1:517231/81‑1 (MQ=255)
aGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATc < 2:517232/81‑1 (MQ=255)
aGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATc < 1:517232/81‑1 (MQ=255)
ggTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGcc > 1:517275/1‑81 (MQ=255)
gTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGccc < 1:517234/81‑1 (MQ=255)
gagaTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCt < 2:517234/80‑1 (MQ=255)
aTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCt > 2:517281/1‑81 (MQ=255)
aTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCt > 2:517262/1‑81 (MQ=255)
tCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGc > 1:517278/1‑81 (MQ=255)
cATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCg > 2:517284/1‑81 (MQ=255)
aTCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCgg < 1:517236/81‑1 (MQ=255)
tCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCgg > 1:517272/1‑80 (MQ=255)
cGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGga > 1:517294/1‑81 (MQ=255)
gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat > 2:517286/1‑81 (MQ=255)
gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat > 2:517289/1‑81 (MQ=255)
aaCGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGt > 2:517273/1‑81 (MQ=255)
aCGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTc < 2:517236/81‑1 (MQ=255)
gAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGc > 2:517269/1‑81 (MQ=255)
gAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGc > 2:517282/1‑81 (MQ=255)
aaCCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGcc < 2:517237/81‑1 (MQ=255)
aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata < 2:517238/81‑1 (MQ=255)
aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat > 1:517284/1‑80 (MQ=255)
aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat > 1:517289/1‑80 (MQ=255)
|
CCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATA > NC_000913/3123226‑3123381
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
