Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,123,304 |
C→T |
A168T (GCC→ACC) |
glcB ← |
malate synthase G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,123,304 | 0 | C | T | 100.0%
| 145.2
/ NA
| 41 | A168T (GCC→ACC) | glcB | malate synthase G |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/23); total (18/23) |
CACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTAT > NC_000913/3123227‑3123380
|
caccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGAt < 2:576395/81‑1 (MQ=255)
aaCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGc > 2:576458/1‑81 (MQ=255)
gCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCa > 2:576473/1‑81 (MQ=255)
caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCAcc > 1:576462/1‑81 (MQ=255)
accacATCCTGATAGCTGCCGTTTTCCAGCAGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACcg < 2:576396/81‑1 (MQ=255)
acaTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGt < 1:576397/81‑1 (MQ=255)
caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGtt < 1:576398/81‑1 (MQ=255)
cTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCgg < 2:576399/81‑1 (MQ=255)
aGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCg > 2:576447/1‑80 (MQ=255)
gCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGt < 2:576402/80‑1 (MQ=255)
gCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGt < 2:576401/80‑1 (MQ=255)
gCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTa < 2:576400/81‑1 (MQ=255)
cTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAg < 2:576403/81‑1 (MQ=255)
tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGc > 1:576468/1‑81 (MQ=255)
ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCg < 2:576404/81‑1 (MQ=255)
gTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCt > 2:576478/1‑81 (MQ=255)
gTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCt < 1:576405/81‑1 (MQ=255)
tCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGAc > 2:576455/1‑80 (MQ=255)
cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt > 1:576489/1‑81 (MQ=255)
cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt > 1:576461/1‑81 (MQ=255)
agagATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCt > 1:576475/1‑81 (MQ=255)
gagaTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCtt < 1:576406/81‑1 (MQ=255)
tCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGc > 1:576465/1‑81 (MQ=255)
cATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGc < 1:576408/80‑1 (MQ=255)
aTCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCgg > 2:576488/1‑81 (MQ=255)
gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat < 1:576410/81‑1 (MQ=255)
gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat > 1:576471/1‑81 (MQ=255)
gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat < 1:576411/81‑1 (MQ=255)
gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat < 2:576409/81‑1 (MQ=255)
aGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatg < 1:576412/81‑1 (MQ=255)
gCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGc > 2:576479/1‑81 (MQ=255)
gCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGc > 2:576480/1‑81 (MQ=255)
ccGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCt > 2:576459/1‑81 (MQ=255)
aCCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCg < 2:576413/81‑1 (MQ=255)
cccAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt < 1:576415/81‑1 (MQ=255)
cccAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt < 2:576414/81‑1 (MQ=255)
ccAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta < 2:576416/81‑1 (MQ=255)
cAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat < 1:576418/81‑1 (MQ=255)
cAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat < 1:576417/81‑1 (MQ=255)
cAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta > 1:576500/1‑80 (MQ=255)
aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTACCTGCGGGATGATGTCGCTGCCGtat > 2:887278/1‑80 (MQ=255)
|
CACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTAT > NC_000913/3123227‑3123380
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
