Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,195,798 |
C→T |
G319D (GGC→GAC) |
rfaE ← |
fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,195,798 | 0 | C | T | 100.0%
| 206.0
/ NA
| 56 | G319D (GGC→GAC) | rfaE | fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/28); total (28/28) |
GTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGCCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCAC > NC_000913/3195720‑3195874
|
gTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCt < 2:589689/81‑1 (MQ=255)
gTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCt < 2:589688/81‑1 (MQ=255)
atcaCCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGt < 1:589690/80‑1 (MQ=255)
accacTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCt < 1:589691/81‑1 (MQ=255)
accacTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCt > 2:589764/1‑81 (MQ=255)
cTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCAc < 2:589692/81‑1 (MQ=255)
cTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCAc > 1:589775/1‑81 (MQ=255)
tttCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGt < 1:589693/80‑1 (MQ=255)
ttCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTc > 2:589786/1‑80 (MQ=255)
tCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTcc < 1:589694/80‑1 (MQ=255)
tCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTcc < 1:589695/80‑1 (MQ=255)
accacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCAc < 1:589696/80‑1 (MQ=255)
ccacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACg > 2:589757/1‑80 (MQ=255)
ccacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACg > 1:589720/1‑80 (MQ=255)
ccacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGt < 2:589697/81‑1 (MQ=255)
cacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTa > 2:589793/1‑81 (MQ=255)
cacGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTa > 2:589746/1‑81 (MQ=255)
acGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTa > 1:589774/1‑80 (MQ=255)
tttACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGc < 2:589698/81‑1 (MQ=255)
ttACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCa > 1:589769/1‑81 (MQ=255)
cgcgCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCAtt < 1:589699/80‑1 (MQ=255)
gcTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTc > 1:589782/1‑80 (MQ=255)
cTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCa < 2:589700/81‑1 (MQ=255)
ccGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAg < 2:589701/79‑1 (MQ=255)
cGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTc < 1:589703/81‑1 (MQ=255)
cGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTc < 2:589702/81‑1 (MQ=255)
gCTACGGCCAGCTTCAGTTCCTCTTTGGTCATCACGCCAAAGTCTGTATCCGCACGTCCACGTATAGCATTTTCCAGCTc > 1:589771/1‑80 (MQ=255)
cTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGa < 1:589705/81‑1 (MQ=255)
cTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGa < 2:589704/81‑1 (MQ=255)
tACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGAt > 1:589763/1‑81 (MQ=255)
cGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGAt < 1:589708/79‑1 (MQ=255)
cGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATc < 1:589707/80‑1 (MQ=255)
cGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCg < 1:589706/81‑1 (MQ=255)
gCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGaaa > 2:589787/1‑81 (MQ=255)
gCTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGaaa > 2:589784/1‑81 (MQ=255)
cTTCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAAc < 2:589709/81‑1 (MQ=255)
tCAGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCATGTCCACGCACAGCATTTTCCAGCTCGATCGGCGAAACCg > 2:589762/1‑81 (MQ=255)
aGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTg > 2:589792/1‑81 (MQ=255)
aGTTCCTCTTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTg > 2:589785/1‑81 (MQ=255)
ctTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTc > 2:589783/1‑81 (MQ=255)
ctTCGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTc > 2:589772/1‑81 (MQ=255)
cGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTccc < 1:589711/80‑1 (MQ=255)
cGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTcc > 2:589778/1‑79 (MQ=255)
cGGTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTc < 1:589710/78‑1 (MQ=255)
ggTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCa < 1:589712/80‑1 (MQ=255)
ggTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAg > 1:589768/1‑81 (MQ=255)
ggTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAg > 1:589770/1‑81 (MQ=255)
gTCATCACGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGt > 2:589761/1‑81 (MQ=255)
atcaCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTg > 2:589790/1‑81 (MQ=255)
caCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc > 2:589779/1‑81 (MQ=255)
caCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc > 2:589760/1‑81 (MQ=255)
caCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc > 1:589789/1‑81 (MQ=255)
aCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGcc < 1:589715/80‑1 (MQ=255)
aCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCg < 2:589713/81‑1 (MQ=255)
aCGCCAAAGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCg < 1:589714/81‑1 (MQ=255)
aaaGTCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGaccac > 2:589781/1‑81 (MQ=255)
|
GTCATCACCACTTTTTCACCACGTTTACGCGCTGCCGCTACGGCCAGCTTCAGTTCCTCTTCGGTCATCACGCCAAAGCCTGTATCTGCACGTCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCAC > NC_000913/3195720‑3195874
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
