Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,340,040 |
A→C |
V9G (GTC→GGC) |
mlaF ← |
intermembrane phospholipid transport system, ATP binding subunit MlaF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,340,040 | 0 | A | C | 100.0%
| 152.5
/ NA
| 43 | V9G (GTC→GGC) | mlaF | intermembrane phospholipid transport system, ATP binding subunit MlaF |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (17/26); total (17/26) |
CCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAC > NC_000913/3339964‑3340116
|
cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCt < 1:623603/79‑1 (MQ=255)
cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCt < 1:623602/79‑1 (MQ=255)
cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCt < 2:623604/79‑1 (MQ=255)
cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTa < 2:623605/80‑1 (MQ=255)
cccTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTa < 2:623601/80‑1 (MQ=255)
tCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAAtt > 2:623668/1‑81 (MQ=255)
gcgGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTc < 2:623606/80‑1 (MQ=255)
ggCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGc > 1:623664/1‑80 (MQ=255)
gCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCca > 2:623691/1‑81 (MQ=255)
cACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCcac < 2:623607/81‑1 (MQ=255)
ggTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGa > 2:623643/1‑81 (MQ=255)
tCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACt < 1:623608/81‑1 (MQ=255)
cAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTg > 2:623663/1‑81 (MQ=255)
gggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCt < 2:623609/81‑1 (MQ=255)
gggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCt > 1:623640/1‑81 (MQ=255)
gAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTcc < 1:623610/81‑1 (MQ=255)
gAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTc > 1:623648/1‑80 (MQ=255)
tattatCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATaa > 1:623656/1‑81 (MQ=255)
ttatCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAAt < 1:623611/80‑1 (MQ=255)
tCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCa < 2:623612/80‑1 (MQ=255)
tCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAc > 1:623660/1‑81 (MQ=255)
tCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAc > 2:623653/1‑81 (MQ=255)
gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAccc < 2:623613/81‑1 (MQ=255)
gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCAccc < 1:623614/81‑1 (MQ=255)
aaGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCt < 1:623615/81‑1 (MQ=255)
aGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCtt < 1:623616/81‑1 (MQ=255)
tGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGt < 2:623617/81‑1 (MQ=255)
tGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGt < 1:623618/81‑1 (MQ=255)
cAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTc < 2:623619/80‑1 (MQ=255)
cAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCt > 2:623665/1‑81 (MQ=255)
aTTGCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGt > 2:623669/1‑81 (MQ=255)
gCCACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGa > 2:623647/1‑81 (MQ=255)
ccACGCGTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGa < 2:623620/80‑1 (MQ=255)
cgTAAAACTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCt < 1:623621/81‑1 (MQ=255)
aaaaCTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAg > 2:623658/1‑81 (MQ=255)
aaaCTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAg > 2:623655/1‑80 (MQ=255)
aCTGACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCAt < 1:623622/81‑1 (MQ=255)
gACATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGgc > 2:623657/1‑81 (MQ=255)
aCATCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGgc < 1:623623/80‑1 (MQ=255)
aTCGCGCATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCt < 2:623624/81‑1 (MQ=255)
cgcATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAt < 1:623625/81‑1 (MQ=255)
cgcATATCGCCTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAt < 1:623626/81‑1 (MQ=255)
atCGCCTAAATTCGCCACAGACTGCTCCATAATTCACTCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAc > 2:623667/1‑81 (MQ=255)
|
CCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAC > NC_000913/3339964‑3340116
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
