Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,594,418 |
C→G |
V355L (GTG→CTG) |
livM ← |
branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,594,418 | 0 | C | G | 100.0%
| 198.5
/ NA
| 54 | V355L (GTG→CTG) | livM | branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (27/27); total (27/27) |
CGCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCACCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAA > NC_000913/3594342‑3594493
|
cGCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACt < 2:684900/81‑1 (MQ=255)
gCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTa > 2:684959/1‑81 (MQ=255)
tCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCg < 2:684901/81‑1 (MQ=255)
aaCTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGc < 1:684902/80‑1 (MQ=255)
cgcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcac > 2:684981/1‑80 (MQ=255)
cgcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcac > 2:684964/1‑80 (MQ=255)
cgcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcac > 1:685006/1‑80 (MQ=255)
gcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGcaca > 1:684996/1‑80 (MQ=255)
gcgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACaa < 1:684903/81‑1 (MQ=255)
cgACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACaaa > 2:684973/1‑81 (MQ=255)
gACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAAc > 2:684988/1‑81 (MQ=255)
gACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAAc < 2:684904/81‑1 (MQ=255)
caccacCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGc > 2:684984/1‑81 (MQ=255)
caGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTc < 2:684905/81‑1 (MQ=255)
aGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCg > 2:684997/1‑81 (MQ=255)
aaaaTTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCa < 1:684906/81‑1 (MQ=255)
aaaTTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCaa < 1:684907/81‑1 (MQ=255)
aTTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAg > 1:684980/1‑81 (MQ=255)
tGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGt > 1:685000/1‑81 (MQ=255)
ccgccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTg < 2:684908/80‑1 (MQ=255)
cgccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTg > 1:685063/1‑79 (MQ=255)
cgccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGaa < 1:684909/81‑1 (MQ=255)
gccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAg > 1:684983/1‑81 (MQ=255)
ccAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAgg < 2:684910/81‑1 (MQ=255)
cAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGa > 2:684982/1‑81 (MQ=255)
cAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGAGAAGGa > 2:684972/1‑81 (MQ=255)
aGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGAt < 2:684911/81‑1 (MQ=255)
gAATCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGAtt > 2:684995/1‑81 (MQ=255)
aTCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTcc < 1:684913/81‑1 (MQ=255)
aTCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTcc < 1:684912/81‑1 (MQ=255)
tCACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCg < 1:684914/81‑1 (MQ=255)
cACCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCgg > 2:684987/1‑81 (MQ=255)
aCCGCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCggg > 2:684990/1‑81 (MQ=255)
gCAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCt > 2:684985/1‑80 (MQ=255)
cAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGa > 2:684978/1‑81 (MQ=255)
cAAATTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGa < 1:684915/81‑1 (MQ=255)
aaaTTGCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGa > 1:684974/1‑80 (MQ=255)
gCGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGc > 2:684998/1‑81 (MQ=255)
cGAGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGc < 2:684916/80‑1 (MQ=255)
aGCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCt < 1:684917/81‑1 (MQ=255)
gCCCATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTg < 1:684918/81‑1 (MQ=255)
ccATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAc < 2:684919/81‑1 (MQ=255)
ccATACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAc < 1:684920/81‑1 (MQ=255)
aTACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAcgc > 1:685014/1‑81 (MQ=255)
tACCGCCGAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGAcgcg < 1:684921/81‑1 (MQ=255)
ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACgcgc < 1:684923/80‑1 (MQ=255)
ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACgcgc < 1:684922/80‑1 (MQ=255)
ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgcc < 2:684925/81‑1 (MQ=255)
ccgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgcc < 1:684924/81‑1 (MQ=255)
cgccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgccg > 2:685008/1‑81 (MQ=255)
gccgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGcgccgc < 2:684926/81‑1 (MQ=255)
cgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCa > 1:684991/1‑80 (MQ=255)
cgAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCa > 2:684994/1‑80 (MQ=255)
gAGCAGCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCaaa > 1:685003/1‑81 (MQ=255)
|
CGCATCAACTCGCGCGACACCACCAGCAAAATTGCCGCCAGAATCACCGCAAATTGCGAGCCCATACCGCCGAGCACCACTATCGCCAGCACAAACGCCGATTCGGCAAAGGTGAAGGATTCCGGGCTGACAAAGCCCTGACGCGCCGCAAA > NC_000913/3594342‑3594493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
