Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,475,523 |
G→A |
W29* (TGG→TGA) |
yjgL → |
protein YjgL |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,475,523 | 0 | G | A | 100.0%
| 61.3
/ NA
| 18 | W29* (TGG→TGA) | yjgL | protein YjgL |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/10); total (8/10) |
AATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATT > NC_000913/4475445‑4475600
|
aaTATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACg > 1:904113/1‑81 (MQ=255)
tatCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACgtg < 2:904089/81‑1 (MQ=255)
aGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTg < 2:904090/80‑1 (MQ=255)
aaCACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGccc < 1:904091/81‑1 (MQ=255)
acacATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCg > 1:904108/1‑81 (MQ=255)
acattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGcc > 2:904115/1‑81 (MQ=255)
cattaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGcc < 2:904092/80‑1 (MQ=255)
ttaGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCggg > 2:904109/1‑81 (MQ=255)
aaTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGAt < 1:904093/81‑1 (MQ=255)
aaTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGAt < 1:904094/81‑1 (MQ=255)
aTTTTAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGAt > 1:904118/1‑80 (MQ=255)
tttAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATaa > 1:904110/1‑80 (MQ=255)
tAAACAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATaa > 1:904117/1‑78 (MQ=255)
aaaCAAAAAAGTGAAGTTTTAAATACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAg < 1:904095/80‑1 (MQ=255)
aGTTTTAAATACCTGACGTGTTGGAATGAATAATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATAttctttc < 2:904096/81‑1 (MQ=255)
aaTACCTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTgg < 1:904097/80‑1 (MQ=255)
ccTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAt > 2:904124/1‑81 (MQ=255)
cTGACGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCAtt < 2:904098/81‑1 (MQ=255)
|
AATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATT > NC_000913/4475445‑4475600
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
