Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,477,066 |
G→A |
V544I (GTT→ATT) |
yjgL → |
protein YjgL |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,477,066 | 0 | G | A | 100.0%
| 58.1
/ NA
| 17 | V544I (GTT→ATT) | yjgL | protein YjgL |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/4); total (13/4) |
ATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAG > NC_000913/4476992‑4477138
|
aTAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCa < 1:856700/81‑1 (MQ=255)
tAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTCGAAGAATCAACCAGATAATATTATCa > 2:856726/1‑80 (MQ=255)
aaaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGaaa > 1:856714/1‑80 (MQ=255)
aaaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTGTGAAGAATCAACCAGATAATATTATCAAAGaaa < 2:856700/80‑1 (MQ=255)
aaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAAc > 1:856712/1‑81 (MQ=255)
tgataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATa > 1:856736/1‑81 (MQ=255)
ataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACt > 1:856717/1‑79 (MQ=255)
aaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACt > 1:856730/1‑77 (MQ=255)
aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGa < 1:856702/80‑1 (MQ=255)
gAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAtt > 1:856720/1‑80 (MQ=255)
aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAtt > 1:856719/1‑79 (MQ=255)
aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAtt > 1:856715/1‑79 (MQ=255)
gAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCa > 2:856720/1‑80 (MQ=255)
aagaaTCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTg > 2:856721/1‑81 (MQ=255)
gaaTCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGc > 2:856732/1‑80 (MQ=255)
aaCCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATc > 1:856738/1‑79 (MQ=255)
cAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGagag < 2:856702/81‑1 (MQ=255)
|
ATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAG > NC_000913/4476992‑4477138
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
