Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
502 |
34.8 |
1912488 |
98.2% |
1878063 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,503,704 |
A→G |
intergenic (‑273/‑354) |
insG ← / → yjhB |
KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,503,704 | 0 | A | G | 100.0%
| 54.9
/ NA
| 16 | intergenic (‑273/‑354) | insG/yjhB | KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/6); total (10/6) |
TAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCA > NC_000913/4503637‑4503771
|
taAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCttt < 1:910409/81‑1 (MQ=255)
taAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCttt > 1:910438/1‑81 (MQ=255)
aGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCt > 2:910442/1‑81 (MQ=255)
ggTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTc < 1:910410/81‑1 (MQ=255)
ataCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAtt > 2:910435/1‑81 (MQ=255)
taCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAttt < 1:910411/81‑1 (MQ=255)
taCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAtt < 1:910412/80‑1 (MQ=255)
aGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGAc < 1:910413/81‑1 (MQ=255)
aGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGAc > 2:910445/1‑81 (MQ=255)
gAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGAc > 1:910437/1‑80 (MQ=255)
aaaaGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACg > 1:910433/1‑80 (MQ=255)
gCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAgg > 1:910441/1‑80 (MQ=255)
aCAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGAc > 2:910439/1‑81 (MQ=255)
cTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTAc > 1:910447/1‑80 (MQ=255)
cTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACg < 2:910414/81‑1 (MQ=255)
gCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGca > 1:910436/1‑80 (MQ=255)
|
TAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCA > NC_000913/4503637‑4503771
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
