Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,883,895 |
G→A |
Q82* (CAA→TAA) |
casA ← |
type I‑E CRISPR system Cascade subunit CasA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,883,895 | 0 | G | A | 100.0%
| 136.3
/ NA
| 38 | Q82* (CAA→TAA) | casA | type I‑E CRISPR system Cascade subunit CasA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/18); total (20/18) |
TTTGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTGTTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGATA > NC_000913/2883818‑2883968
|
tttGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAgttatt > 2:523220/1‑80 (MQ=255)
tGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGa < 1:523186/80‑1 (MQ=255)
gACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGaa < 2:523187/80‑1 (MQ=255)
gACACCTTTGGTCTGCAT‑AAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAAc > 1:523231/1‑81 (MQ=255)
cacCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACt > 2:523222/1‑81 (MQ=255)
ttGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCt > 2:523240/1‑81 (MQ=255)
gTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTc < 2:523188/80‑1 (MQ=255)
gCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGt > 1:523235/1‑79 (MQ=255)
cATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGt < 1:523189/78‑1 (MQ=255)
aTAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGc < 2:523190/81‑1 (MQ=255)
aaGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGa > 1:523228/1‑81 (MQ=255)
gggATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGAtt > 2:523237/1‑81 (MQ=255)
ggATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTc < 2:523191/81‑1 (MQ=255)
cTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGc > 1:523233/1‑81 (MQ=255)
cGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGATATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGAt < 1:523192/81‑1 (MQ=255)
aTTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTc > 1:523221/1‑80 (MQ=255)
ttAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGa < 1:523193/81‑1 (MQ=255)
aaGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGaaa > 1:523226/1‑81 (MQ=255)
aaGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGaaa > 1:523232/1‑81 (MQ=255)
ggTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAAtt < 1:523195/81‑1 (MQ=255)
ggTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAAtt < 2:523194/81‑1 (MQ=255)
gTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTc < 1:523196/81‑1 (MQ=255)
aGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCaa > 2:523248/1‑81 (MQ=255)
gAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAAc > 1:523242/1‑81 (MQ=255)
atatCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCa > 2:523229/1‑81 (MQ=255)
tatctatcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCAt > 1:523245/1‑81 (MQ=255)
atctatcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATAATGCGATGTCGAAATTCAACGTCATc > 2:523244/1‑81 (MQ=255)
tctatcCACGTCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATc < 1:523197/80‑1 (MQ=255)
ctatcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCtt > 2:523250/1‑81 (MQ=255)
atcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCtttt > 1:523238/1‑81 (MQ=255)
tcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCtt < 2:523198/78‑1 (MQ=255)
tcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCt < 1:523199/77‑1 (MQ=255)
ccACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGc > 1:523236/1‑81 (MQ=255)
cACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGcc < 2:523200/81‑1 (MQ=255)
ggCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCggg > 2:523225/1‑81 (MQ=255)
gcgATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCg < 2:523201/81‑1 (MQ=255)
gATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGAt < 2:523202/81‑1 (MQ=255)
aTGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGAt > 1:523256/1‑80 (MQ=255)
aTGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGATa < 2:523203/81‑1 (MQ=255)
|
TTTGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTGTTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGATA > NC_000913/2883818‑2883968
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
