Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,891,810 |
G→A |
P30L (CCC→CTC) |
cysJ ← |
sulfite reductase, flavoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,891,810 | 0 | G | A | 100.0%
| 111.3
/ NA
| 31 | P30L (CCC→CTC) | cysJ | sulfite reductase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/21); total (10/21) |
CTGGCGTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGGGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGAAGGTGGGA > NC_000913/2891733‑2891885
|
ctggcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGagag > 2:524840/1‑81 (MQ=255)
gcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGtt < 2:524802/80‑1 (MQ=255)
gcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGt < 1:524801/79‑1 (MQ=255)
gcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTa < 1:524803/81‑1 (MQ=255)
cgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTaa < 1:524804/81‑1 (MQ=255)
gTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTaaa < 1:524805/81‑1 (MQ=255)
gTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTaaa > 1:524852/1‑81 (MQ=255)
aaGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGt < 1:524807/81‑1 (MQ=255)
aaGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGt < 2:524806/81‑1 (MQ=255)
gcagcaGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGgcc < 1:524808/81‑1 (MQ=255)
cagcagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGgccg < 1:524809/81‑1 (MQ=255)
cagcagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGgccg < 2:524810/81‑1 (MQ=255)
cagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCt < 1:524812/81‑1 (MQ=255)
cagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCt < 2:524811/81‑1 (MQ=255)
agGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCt > 1:524847/1‑80 (MQ=255)
gctgctGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTg > 1:524841/1‑79 (MQ=255)
tgATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGt > 1:524860/1‑81 (MQ=255)
aTTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGt < 1:524813/79‑1 (MQ=255)
gAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAg > 2:524849/1‑81 (MQ=255)
aCGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGc > 1:524851/1‑81 (MQ=255)
ccAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCgg > 1:524846/1‑81 (MQ=255)
gAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGtt < 1:524814/81‑1 (MQ=255)
aaaTAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGtt > 2:524850/1‑80 (MQ=255)
aaTAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCa < 2:524815/81‑1 (MQ=255)
tAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAAc < 2:524816/81‑1 (MQ=255)
gCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACgg < 1:524817/81‑1 (MQ=255)
ccAGAAACCCAGGCAAGCTGGGTGAGAGTTAGATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGa < 2:524818/81‑1 (MQ=255)
aGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAg > 1:524853/1‑81 (MQ=255)
aaaCCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGc > 2:524855/1‑80 (MQ=255)
ggCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGa < 1:524820/81‑1 (MQ=255)
ggCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGa < 1:524819/81‑1 (MQ=255)
ggCAAGCTGGGTGAGAGTAAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGa < 2:524821/81‑1 (MQ=255)
gggtgagAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGAAGGTGGGa > 1:524854/1‑81 (MQ=255)
|
CTGGCGTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGGGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGAAGGTGGGA > NC_000913/2891733‑2891885
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
