Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,967,332 |
Δ1 bp |
coding (1103/2247 nt) |
ptsP ← |
phosphoenolpyruvate‑protein phosphotransferase PtsP |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,967,331 | 0 | G | . | 100.0%
| 180.6
/ NA
| 43 | coding (1104/2247 nt) | ptsP | phosphoenolpyruvate‑protein phosphotransferase PtsP |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (22/21); total (22/21) |
GCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACT > NC_000913/2967255‑2967406
|
gCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACgtagg < 1:541370/81‑5 (MQ=255)
cACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATccc > 2:541441/1‑81 (MQ=255)
cgcgATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTTGGGATCCTCAg > 2:541437/1‑81 (MQ=255)
cgcgATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAg > 1:541428/1‑81 (MQ=255)
gATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAgcg > 1:541430/1‑80 (MQ=255)
aTAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCa > 1:541424/1‑81 (MQ=255)
tAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCAc < 1:541371/81‑1 (MQ=255)
gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACg < 1:541372/80‑1 (MQ=255)
gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGt < 1:541373/81‑1 (MQ=255)
ccATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTa < 1:541374/81‑1 (MQ=255)
cATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTAc > 1:541429/1‑81 (MQ=255)
aaCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCAt < 2:541375/80‑1 (MQ=255)
aaCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCAt > 1:541455/1‑80 (MQ=255)
aaCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCAt > 1:541405/1‑80 (MQ=255)
aCGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGa < 1:541376/81‑1 (MQ=255)
cAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCg > 2:541447/1‑79 (MQ=255)
aGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCgcag > 2:541444/1‑81 (MQ=255)
gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCAt > 2:541467/1‑81 (MQ=255)
tCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATg < 1:541377/81‑1 (MQ=255)
cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATggg < 2:541378/81‑1 (MQ=255)
cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATgg > 1:541439/1‑80 (MQ=255)
cGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGt > 2:541462/1‑81 (MQ=255)
gATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGtt < 2:541379/81‑1 (MQ=255)
aTGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTg < 2:541380/81‑1 (MQ=255)
gcagaaGCGAAGGCTGAATATCCGCGAGCAGCAC‑GTAGGGATACCCAGAGAACGTAACATGATCGAAGAATGGGAGTTGGc < 2:541385/81‑1 (MQ=255)
cagcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGc < 1:541382/80‑1 (MQ=255)
cagcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCt < 2:541381/81‑1 (MQ=255)
cagcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCt < 1:541383/81‑1 (MQ=255)
caCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTgcg < 2:541384/81‑1 (MQ=255)
gAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCAt > 2:541450/1‑81 (MQ=255)
ggCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCAtctc < 1:541386/81‑1 (MQ=255)
tGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCa > 1:541456/1‑81 (MQ=255)
aaTATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCAc < 1:541387/80‑1 (MQ=255)
tatCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac < 2:541388/81‑1 (MQ=255)
atCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac > 1:541446/1‑80 (MQ=255)
cgcgCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaaca > 1:541443/1‑81 (MQ=255)
cgcgCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGacaaca > 2:541448/1‑81 (MQ=255)
gcgcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAacac > 2:541463/1‑81 (MQ=255)
cgcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAcc < 2:541389/81‑1 (MQ=255)
gcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACAcc > 2:671095/1‑80 (MQ=255)
gcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCg < 2:541391/81‑1 (MQ=255)
gcCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCg < 1:541390/81‑1 (MQ=255)
cccATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGa > 2:541438/1‑81 (MQ=255)
aTCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACt > 2:541432/1‑80 (MQ=255)
|
GCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACT > NC_000913/2967255‑2967406
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
