Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,060,772 |
(T)8→9 |
intergenic (+126/‑78) |
argP → / → scpA |
DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,060,764 | 1 | . | T | 100.0%
| 99.6
/ NA
| 28 | intergenic (+118/‑86) | argP/scpA | DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (15/13); total (15/13) |
CTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAC > NC_000913/3060694‑3060839
|
cttTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑tttttttttg > 2:561625/1‑79 (MQ=255)
ttCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACa < 2:561582/81‑1 (MQ=255)
ccTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACaaa > 2:561618/1‑81 (MQ=255)
cTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAAc < 1:561583/81‑1 (MQ=255)
gAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAAcc < 1:561584/79‑1 (MQ=255)
gAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCa > 2:561604/1‑81 (MQ=255)
tcacatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACg > 2:561638/1‑80 (MQ=255)
cacatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGt > 1:561611/1‑80 (MQ=255)
acatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTa > 1:561616/1‑80 (MQ=255)
acaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAg < 1:561585/80‑1 (MQ=255)
aaaTAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCa < 1:561586/81‑1 (MQ=255)
aTAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCAcc < 2:561587/81‑1 (MQ=255)
tAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCg < 2:561588/81‑1 (MQ=255)
gACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGt < 1:561589/80‑1 (MQ=255)
aCAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAg > 2:561624/1‑81 (MQ=255)
aaaTCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGgc < 1:561591/81‑1 (MQ=255)
aaaTCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGgc < 1:561590/81‑1 (MQ=255)
ctcAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCa > 1:561614/1‑80 (MQ=255)
cAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCaaa > 2:561608/1‑80 (MQ=255)
aGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCaa < 2:561592/78‑1 (MQ=255)
aGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATa > 2:561634/1‑81 (MQ=255)
gcggcAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATAcc < 1:561593/81‑1 (MQ=255)
ggcAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCt > 1:561629/1‑81 (MQ=255)
gcAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTc < 1:561594/81‑1 (MQ=255)
cAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCa > 1:561626/1‑81 (MQ=255)
aaaaaaCGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCAt > 2:561630/1‑81 (MQ=255)
gacgTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGAtt > 2:561621/1‑81 (MQ=255)
cgTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGc > 1:561620/1‑81 (MQ=255)
aTGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAc > 2:561615/1‑81 (MQ=255)
|
CTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAC > NC_000913/3060694‑3060839
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
