Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,064,651 |
A→G |
H220R (CAC→CGC) |
scpB → |
methylmalonyl‑CoA decarboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,064,651 | 0 | A | G | 100.0%
| 135.2
/ NA
| 39 | H220R (CAC→CGC) | scpB | methylmalonyl‑CoA decarboxylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/25); total (14/25) |
CACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGG > NC_000913/3064578‑3064725
|
caccacATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCAt > 1:504728/1‑80 (MQ=255)
caccacATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCAt > 2:504732/1‑80 (MQ=255)
cacaTCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAAc > 2:504733/1‑81 (MQ=255)
acaTCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGCGCTGCGTGTACTGGGCGAAGCACGCACCATGAACt < 2:504678/81‑1 (MQ=255)
aTCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTcc < 1:504680/81‑1 (MQ=255)
gagaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGaa > 2:504738/1‑81 (MQ=255)
agaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATg < 1:504681/78‑1 (MQ=255)
agaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGa < 1:504682/79‑1 (MQ=255)
gaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAAtt < 2:504682/81‑1 (MQ=255)
aaaGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAAttt < 2:504683/81‑1 (MQ=255)
aaaGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAAttt > 1:504725/1‑81 (MQ=255)
aGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGa < 1:504685/81‑1 (MQ=255)
ccGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGt < 2:504685/81‑1 (MQ=255)
ccATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAgg < 2:504687/81‑1 (MQ=255)
ccATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAgg < 1:504687/81‑1 (MQ=255)
cATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAggg < 1:504689/81‑1 (MQ=255)
cATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAggg > 1:504735/1‑81 (MQ=255)
gCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATg > 1:504749/1‑81 (MQ=255)
ccGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATgc < 2:504691/81‑1 (MQ=255)
ccGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATgc < 1:504691/81‑1 (MQ=255)
ccGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATg < 2:504689/80‑1 (MQ=255)
ttATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGcgcc > 2:504731/1‑81 (MQ=255)
ttATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGcgcc < 1:504693/81‑1 (MQ=255)
aTCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGcgccg < 2:504693/80‑1 (MQ=255)
gaagaGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGgtg < 2:504694/81‑1 (MQ=255)
aagaGCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGgtg > 2:504735/1‑80 (MQ=255)
gagCTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTAt < 1:504696/81‑1 (MQ=255)
cTGCGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGa > 2:504745/1‑80 (MQ=255)
cGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGc < 2:504696/81‑1 (MQ=255)
cGTGTACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGc < 1:504698/81‑1 (MQ=255)
gtACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCg > 1:504738/1‑79 (MQ=255)
gtACTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCg > 2:504744/1‑79 (MQ=255)
cTGGGCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGAt < 1:504699/81‑1 (MQ=255)
gggCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTa > 1:504726/1‑81 (MQ=255)
ggCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTa < 1:504700/80‑1 (MQ=255)
gCGAAGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTAcc < 1:504701/81‑1 (MQ=255)
cGAAGAACGCACAATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCa < 1:504702/81‑1 (MQ=255)
aaGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGATGATTACCAg > 2:504739/1‑80 (MQ=255)
aaGCACGCACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAgg < 1:504703/81‑1 (MQ=255)
|
CACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGG > NC_000913/3064578‑3064725
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
