Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,102,776 |
C→T |
R26H (CGC→CAC) |
trmI ← |
tRNA m(7)G46 methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,102,776 | 0 | C | T | 100.0%
| 136.5
/ NA
| 38 | R26H (CGC→CAC) | trmI | tRNA m(7)G46 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/21); total (17/21) |
CTTCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGCGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGTTTTTC > NC_000913/3102699‑3102850
|
cTTCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTggtggc > 2:571647/1‑81 (MQ=255)
tCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGgtggcg > 2:571645/1‑80 (MQ=255)
cGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCtggtgg < 2:571592/77‑1 (MQ=255)
cGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGca > 1:571649/1‑81 (MQ=255)
gAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACaa > 2:571638/1‑80 (MQ=255)
aaCGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACg > 1:571635/1‑81 (MQ=255)
cGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGa > 2:571644/1‑81 (MQ=255)
gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt < 2:571596/81‑1 (MQ=255)
gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt < 2:571594/81‑1 (MQ=255)
gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTAGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt < 1:571595/81‑1 (MQ=255)
gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTAGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt < 1:571593/81‑1 (MQ=255)
cccATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATa > 1:571634/1‑81 (MQ=255)
cATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACg > 1:571643/1‑81 (MQ=255)
cATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACg < 1:571597/81‑1 (MQ=255)
tCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGgc < 1:571600/81‑1 (MQ=255)
tCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGgc < 2:571599/81‑1 (MQ=255)
tCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGgc < 2:571598/81‑1 (MQ=255)
ggCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGt < 1:571601/81‑1 (MQ=255)
gCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTGTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTg < 1:571602/81‑1 (MQ=255)
agtagtTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGggc < 1:571604/81‑1 (MQ=255)
agtagtTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGggc < 2:571603/81‑1 (MQ=255)
gtagtTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGggcg < 2:571605/81‑1 (MQ=255)
tCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGttt > 2:571637/1‑81 (MQ=255)
ccAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGttt < 2:571606/80‑1 (MQ=255)
cAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGtttt < 1:571607/80‑1 (MQ=255)
cAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGtttt > 1:571655/1‑80 (MQ=255)
cAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGtttt > 1:571651/1‑80 (MQ=255)
ttCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTc > 1:571681/1‑81 (MQ=255)
ccTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTcc < 2:571608/80‑1 (MQ=255)
ccTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTcc < 2:571609/80‑1 (MQ=255)
cTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCgg > 2:571659/1‑81 (MQ=255)
tGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGt > 1:571652/1‑81 (MQ=255)
gCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTg > 2:571662/1‑80 (MQ=255)
gCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTg > 1:571653/1‑80 (MQ=255)
ccTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGa > 2:571650/1‑80 (MQ=255)
cTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGaaa > 1:571660/1‑81 (MQ=255)
gTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGAcgtcgt < 2:571610/81‑1 (MQ=255)
gCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGtttt > 2:571657/1‑81 (MQ=255)
gCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGttt < 1:571611/80‑1 (MQ=255)
gCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGttt < 1:571612/80‑1 (MQ=255)
cccTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGTTTTTc < 2:571613/81‑1 (MQ=255)
|
CTTCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGCGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGTTTTTC > NC_000913/3102699‑3102850
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
