Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,139,574 |
A→T |
E283V (GAA→GTA) |
yghU → |
disulfide reductase/organic hydroperoxide reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,139,574 | 0 | A | T | 100.0%
| 135.5
/ NA
| 38 | E283V (GAA→GTA) | yghU | disulfide reductase/organic hydroperoxide reductase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (22/16); total (22/16) |
GTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAT > NC_000913/3139498‑3139649
|
gTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGa < 1:580407/80‑1 (MQ=255)
aCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGc > 2:580443/1‑81 (MQ=255)
cGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGt > 2:580483/1‑81 (MQ=255)
aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAg < 1:580409/81‑1 (MQ=255)
ccAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgg < 2:580410/81‑1 (MQ=255)
aaCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgggg > 1:580459/1‑81 (MQ=255)
aaCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgggg > 2:580451/1‑81 (MQ=255)
aCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgggg > 2:580455/1‑80 (MQ=255)
cGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTa < 2:580412/81‑1 (MQ=255)
cGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTa < 2:580411/81‑1 (MQ=255)
ggACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGt > 2:580464/1‑79 (MQ=255)
ggACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGt > 1:580482/1‑79 (MQ=255)
cGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGt > 2:580476/1‑81 (MQ=255)
cGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGt > 1:580477/1‑81 (MQ=255)
gAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgt > 2:580463/1‑81 (MQ=255)
aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgt < 2:580413/80‑1 (MQ=255)
aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTCGgt < 2:580414/80‑1 (MQ=255)
aTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgtgt < 1:580416/81‑1 (MQ=255)
aTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgtgt < 2:580415/81‑1 (MQ=255)
aTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgtgt > 1:580475/1‑81 (MQ=255)
gAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtc > 1:580481/1‑81 (MQ=255)
aGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgtcg > 1:580470/1‑81 (MQ=255)
gTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgtcg > 1:580479/1‑80 (MQ=255)
ttGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgtcg > 1:580467/1‑79 (MQ=255)
cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcagc < 1:580417/80‑1 (MQ=255)
aTGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcagc > 1:580474/1‑79 (MQ=255)
gcgaCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCa < 2:580418/81‑1 (MQ=255)
cATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCa < 2:580419/81‑1 (MQ=255)
aTGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAg > 1:580469/1‑81 (MQ=255)
gACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGc > 2:580480/1‑81 (MQ=255)
cAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGAt < 1:580420/81‑1 (MQ=255)
aGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATa < 2:580421/81‑1 (MQ=255)
cGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGtt > 1:580489/1‑80 (MQ=255)
gagaCGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCa > 2:580473/1‑81 (MQ=255)
agaCGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAc < 1:580422/81‑1 (MQ=255)
cGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgcc < 1:580423/81‑1 (MQ=255)
cGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgcc > 1:580488/1‑81 (MQ=255)
tACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAt > 1:580471/1‑81 (MQ=255)
|
GTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAT > NC_000913/3139498‑3139649
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
