Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,219,561 |
A→G |
K23R (AAG→AGG) |
patA → |
putrescine aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,219,561 | 0 | A | G | 100.0%
| 134.7
/ NA
| 40 | K23R (AAG→AGG) | patA | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/25); total (15/25) |
CGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTC > NC_000913/3219483‑3219631
|
cGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGc < 2:599866/81‑1 (MQ=255)
cGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGc < 2:599865/81‑1 (MQ=255)
ggAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCg < 2:599869/81‑1 (MQ=255)
ggAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCg < 2:599868/81‑1 (MQ=255)
ggAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCg < 2:599867/81‑1 (MQ=255)
aGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGaa > 2:599931/1‑81 (MQ=255)
gCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAAc > 1:599914/1‑81 (MQ=255)
atatTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGc > 1:599928/1‑81 (MQ=255)
atTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTg > 1:599941/1‑81 (MQ=255)
ttttGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTg < 1:599870/80‑1 (MQ=255)
ttGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGa < 2:599871/80‑1 (MQ=255)
gAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATc < 1:599872/80‑1 (MQ=255)
aaCAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCAt < 1:599873/81‑1 (MQ=255)
cAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCAt > 1:599926/1‑79 (MQ=255)
aGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATgag < 1:599874/81‑1 (MQ=255)
gTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATgagga < 1:599875/81‑1 (MQ=255)
ttACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATgaggag < 1:599877/81‑1 (MQ=255)
ttACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATgaggag < 2:599876/81‑1 (MQ=255)
ccTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGAt > 1:599936/1‑80 (MQ=255)
gAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAg > 2:599922/1‑80 (MQ=255)
gAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAg > 2:599921/1‑80 (MQ=255)
aTCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTaa > 2:599932/1‑81 (MQ=255)
ggCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAAcc < 1:599878/80‑1 (MQ=255)
ggCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAAcc > 2:599920/1‑80 (MQ=255)
gCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAAcc > 2:599938/1‑79 (MQ=255)
cTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCgag < 2:599879/81‑1 (MQ=255)
gCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTg < 2:599880/81‑1 (MQ=255)
cGTGCAGCGCCCACTCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGa < 2:599881/81‑1 (MQ=255)
agagCAGCGCCCAAGCCATGAATCTCATTGAGAGGCAAACGCTGGATCATGAGGAGATGAAAGCACTTACCCGAGAGGTGa < 2:599882/78‑1 (MQ=255)
gTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGAt > 1:599930/1‑81 (MQ=255)
aGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGaa < 2:599883/81‑1 (MQ=255)
aGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGaa > 2:599937/1‑81 (MQ=255)
gcgcCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAAt < 2:599884/81‑1 (MQ=255)
gCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCaaa < 2:599886/81‑1 (MQ=255)
gCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCaaa < 1:599885/81‑1 (MQ=255)
gCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCa > 2:599943/1‑79 (MQ=255)
cTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAagag > 1:599948/1‑81 (MQ=255)
aaTCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGc < 1:599887/79‑1 (MQ=255)
aTCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATg < 2:599888/81‑1 (MQ=255)
aTTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTc < 1:599889/78‑1 (MQ=255)
|
CGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTC > NC_000913/3219483‑3219631
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
