Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,219,561 |
A→G |
K23R (AAG→AGG) |
patA → |
putrescine aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,219,561 | 0 | A | G | 100.0%
| 78.9
/ NA
| 24 | K23R (AAG→AGG) | patA | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/11); total (13/11) |
CGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTC > NC_000913/3219483‑3219631
|
cGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGc < 2:538586/81‑1 (MQ=255)
ggAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCg < 2:538587/81‑1 (MQ=255)
gCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAAc > 2:538617/1‑81 (MQ=255)
atatTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGc < 2:538588/81‑1 (MQ=255)
tatTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCt > 2:538589/1‑81 (MQ=255)
ttttGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTg > 2:538611/1‑80 (MQ=255)
gAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCa > 2:538623/1‑81 (MQ=255)
aCCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGAt > 1:538620/1‑81 (MQ=255)
ccTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATg < 2:538590/81‑1 (MQ=255)
tCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGa > 2:538625/1‑79 (MQ=255)
gAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAg < 2:538591/80‑1 (MQ=255)
gAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAg > 2:538649/1‑80 (MQ=255)
gAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAg > 1:538617/1‑80 (MQ=255)
cGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAAcc > 1:538621/1‑81 (MQ=255)
ggCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCg < 2:538592/81‑1 (MQ=255)
cTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCgag < 2:538593/81‑1 (MQ=255)
tAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAgg > 2:538618/1‑81 (MQ=255)
cAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTg < 1:538595/80‑1 (MQ=255)
cAGCGCCCACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTg < 2:538595/80‑1 (MQ=255)
cACGCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTc < 1:538597/81‑1 (MQ=255)
gCCCTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCa > 1:538635/1‑79 (MQ=255)
ccTGAATCTCATTGAGAGGCGAACGCTGGGTCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCaaa > 2:538639/1‑79 (MQ=255)
ccTGAATCTCATTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCaaa > 2:538630/1‑79 (MQ=255)
aTTGAGAGGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTc < 1:538598/78‑1 (MQ=255)
|
CGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCTGAATCTCATTGAGAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGCATGTC > NC_000913/3219483‑3219631
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
