Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,265,761 |
C→A |
V90F (GTT→TTT) |
tdcB ← |
catabolic threonine dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,265,761 | 0 | C | A | 100.0%
| 111.5
/ NA
| 31 | V90F (GTT→TTT) | tdcB | catabolic threonine dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/13); total (18/13) |
CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT > NC_000913/3265684‑3265838
|
cGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAaccc > 2:611150/1‑81 (MQ=255)
gCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCt > 1:611167/1‑81 (MQ=255)
gCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCt > 1:611140/1‑81 (MQ=255)
gCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCt > 1:611152/1‑81 (MQ=255)
aCTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTgc > 1:611166/1‑81 (MQ=255)
cTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTg < 2:611114/79‑1 (MQ=255)
gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATgg > 2:611149/1‑81 (MQ=255)
gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATgg < 2:611115/81‑1 (MQ=255)
gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATg < 1:611116/80‑1 (MQ=255)
tGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGcc > 2:611160/1‑81 (MQ=255)
cTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAAc < 1:611117/81‑1 (MQ=255)
tttGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAg > 1:611171/1‑81 (MQ=255)
ttGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAgg > 2:611159/1‑81 (MQ=255)
gCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGcc < 1:611118/80‑1 (MQ=255)
cATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac < 1:611120/81‑1 (MQ=255)
cATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac < 2:611119/81‑1 (MQ=255)
aTCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac > 1:611157/1‑80 (MQ=255)
aTCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGccac > 2:611162/1‑80 (MQ=255)
tttACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCttt > 1:611168/1‑81 (MQ=255)
aCCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGc < 1:611121/80‑1 (MQ=255)
ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGt > 1:611164/1‑81 (MQ=255)
ccGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGt > 1:611173/1‑81 (MQ=255)
cGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGttttt > 2:611163/1‑81 (MQ=255)
cgcgCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg > 2:611165/1‑81 (MQ=255)
cgcgCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg > 1:611170/1‑81 (MQ=255)
gcgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg < 1:611123/80‑1 (MQ=255)
gcgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg < 1:611122/80‑1 (MQ=255)
cAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGaa < 2:611124/81‑1 (MQ=255)
cAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGaa < 1:611125/81‑1 (MQ=255)
agagGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACtt > 2:611156/1‑80 (MQ=255)
ggAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAAttt < 2:611126/81‑1 (MQ=255)
gAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATtta > 2:611180/1‑81 (MQ=255)
aaaaCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATttat < 1:611127/81‑1 (MQ=255)
|
CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT > NC_000913/3265684‑3265838
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
