Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,265,761 |
C→A |
V90F (GTT→TTT) |
tdcB ← |
catabolic threonine dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,265,761 | 0 | C | A | 100.0%
| 114.6
/ NA
| 32 | V90F (GTT→TTT) | tdcB | catabolic threonine dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/16); total (16/16) |
CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT > NC_000913/3265684‑3265838
|
cGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAaccc < 2:549000/81‑1 (MQ=255)
tGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCAt > 1:549042/1‑81 (MQ=255)
ggATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATg > 2:549042/1‑81 (MQ=255)
gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATgg > 1:549035/1‑81 (MQ=255)
gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATg < 2:549001/80‑1 (MQ=255)
aTTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGt < 2:549003/81‑1 (MQ=255)
aTTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGt < 2:549002/81‑1 (MQ=255)
ttttGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGtt < 1:549005/81‑1 (MQ=255)
ttttGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGtt < 2:549005/81‑1 (MQ=255)
gcACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCg > 2:549052/1‑79 (MQ=255)
cACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCg > 2:549039/1‑78 (MQ=255)
cTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGaa > 2:549036/1‑80 (MQ=255)
ttttGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGaa > 1:549039/1‑79 (MQ=255)
cacTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGcc < 2:549006/81‑1 (MQ=255)
cTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCtt < 1:549008/81‑1 (MQ=255)
tttACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCttt > 1:549046/1‑81 (MQ=255)
tACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGc > 1:549061/1‑81 (MQ=255)
gTCGATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGttt > 2:549061/1‑81 (MQ=255)
gATACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTc > 2:549049/1‑81 (MQ=255)
aTACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTcc < 2:549008/81‑1 (MQ=255)
aTACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTcc < 2:549009/81‑1 (MQ=255)
tACCCAGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCg < 2:549010/81‑1 (MQ=255)
aGCATCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCg < 2:549011/81‑1 (MQ=255)
tCGCGCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGt > 1:549055/1‑79 (MQ=255)
cgcgCAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAg > 1:549057/1‑81 (MQ=255)
cgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTg < 1:549014/81‑1 (MQ=255)
cgcAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTg < 1:549013/81‑1 (MQ=255)
cAGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGaa > 1:549047/1‑81 (MQ=255)
aGGAGAGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAAc > 1:549059/1‑81 (MQ=255)
gagaGGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACtt < 1:549015/81‑1 (MQ=255)
gagGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTaa < 1:549016/81‑1 (MQ=255)
agGGAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAAt > 1:549056/1‑81 (MQ=255)
gggAAAACCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAAtt < 1:549017/81‑1 (MQ=255)
aaaaCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATttat < 2:549017/81‑1 (MQ=255)
|
CGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTGCGCATGGTTGCCCGCAGAACAGGCCACCACGCCTTTGCGTTTTTCCGCATCGGTCAGTGAACTTAATTTAT > NC_000913/3265684‑3265838
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
