Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,313,786 |
C→T |
L743L (CTG→CTA) |
infB ← |
translation initiation factor IF‑2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,313,786 | 0 | C | T | 100.0%
| 181.1
/ NA
| 50 | L743L (CTG→CTA) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/24); total (26/24) |
GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATC > NC_000913/3313708‑3313858
|
ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg > 1:622724/1‑81 (MQ=255)
ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg > 2:622717/1‑81 (MQ=255)
ggAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAg > 2:622714/1‑81 (MQ=255)
agtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATgg > 1:622721/1‑81 (MQ=255)
gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGc > 1:622737/1‑81 (MQ=255)
gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGc < 2:622653/81‑1 (MQ=255)
gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGc > 1:622711/1‑81 (MQ=255)
taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCg < 1:622654/81‑1 (MQ=255)
taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCg < 2:622655/81‑1 (MQ=255)
aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGtt < 2:622656/81‑1 (MQ=255)
aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGtt < 2:622657/81‑1 (MQ=255)
aCGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGtt < 2:622658/81‑1 (MQ=255)
gCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTg > 1:622718/1‑80 (MQ=255)
aGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAc < 1:622659/81‑1 (MQ=255)
tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAcgcc < 2:622661/81‑1 (MQ=255)
tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGAcgcc < 1:622660/81‑1 (MQ=255)
gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGc < 1:622662/81‑1 (MQ=255)
tttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGc > 1:622720/1‑79 (MQ=255)
gCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTg > 1:622751/1‑81 (MQ=255)
tCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg > 2:622750/1‑81 (MQ=255)
tCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg > 2:622719/1‑81 (MQ=255)
cAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg < 2:622663/80‑1 (MQ=255)
aaTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTc < 2:622664/81‑1 (MQ=255)
aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGtt > 2:622731/1‑81 (MQ=255)
tttACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTc > 2:622729/1‑81 (MQ=255)
tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCgg > 1:622749/1‑81 (MQ=255)
gCAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATa > 2:622725/1‑81 (MQ=255)
cAGAGGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATac > 2:622741/1‑81 (MQ=255)
agagGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacc < 1:622665/81‑1 (MQ=255)
gagGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacca > 1:622728/1‑81 (MQ=255)
gagGCATCAGCACGAACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATacca > 2:622734/1‑81 (MQ=255)
agGCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac > 1:622738/1‑81 (MQ=255)
ggCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc > 2:622736/1‑81 (MQ=255)
ggCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccacc < 2:622666/81‑1 (MQ=255)
gCATCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCt < 2:622667/81‑1 (MQ=255)
aTCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAc > 1:622747/1‑81 (MQ=255)
tCAGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTAGGTGATACCACCTACg < 1:622668/81‑1 (MQ=255)
aGCACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGc < 1:622669/80‑1 (MQ=255)
cACGTACGTTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAg < 1:622670/81‑1 (MQ=255)
tacgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc < 1:622671/81‑1 (MQ=255)
acgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAAcc > 1:622723/1‑80 (MQ=255)
cgtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCg < 1:622672/80‑1 (MQ=255)
gtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat > 1:622733/1‑81 (MQ=255)
gtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat > 1:622745/1‑81 (MQ=255)
gtTAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgat > 1:622752/1‑81 (MQ=255)
ttAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg < 2:622673/81‑1 (MQ=255)
ttAAAGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg < 2:622674/81‑1 (MQ=255)
aaaGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatgat > 1:622732/1‑81 (MQ=255)
aaGCCAACTAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATc < 2:622675/81‑1 (MQ=255)
aaGCCAACTAGGATGGCGTTGGACGAAGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatgat < 1:622676/80‑1 (MQ=255)
|
GGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATC > NC_000913/3313708‑3313858
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
